Detection of Leishmania infantum and Leishmania amazonensis in Bats From Endemic and Non-endemic Areas of São Paulo State, Brazil

IF 2.4 2区农林科学 Q3 INFECTIOUS DISEASES

Zoonoses and Public Health Pub Date : 2024-12-08 DOI:10.1111/zph.13201

Danilo Alves de França, Sara Zúquete, Mariana Louro, Maíra Guimarães Kersul, Benedito Donizete Menozzi, Felipe Fornazari, Gabriela Santos-Gomes, Isabel Pereira da Fonseca, Helio Langoni

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引用次数: 0

Abstract

Aims

Leishmaniasis is an endemic disease in several regions of Brazil, a tropical country that presents specific environmental conditions that contribute to the development of phlebotomine vectors. This study aimed to detect Leishmania species in naturally infected bats from 17 municipalities in the São Paulo state.

Methods and Results

Spleen and liver samples from 203 bats were analysed by real-time PCR and confirmed by conventional PCR followed by gene sequencing. Leishmania DNA was amplified by real-time PCR in 6.4% of the bats and by conventional PCR followed by sequencing in 3.4% of the bats. Positive samples were characterised and included in GenBank. Leishmania species were confirmed in M. molossus , M. nigricans and E. glaucinus bats. Leishmania (Leishmania) amazonensis and L. infantum (syn. L. chagasi) were identified. This is the first detection of Leishmania spp. in bats in the studied areas. All the positive bats came from urban areas. Insectivorous bats were statistically more positive. There was similarity between our sequences and those of a human isolate and a phlebotomine from the region.

Conclusions

This result points to bats as important possible reservoir of Leishmania in Brazil and guides the country's health authorities towards epidemiological surveillance, control and prevention actions in endemic areas.

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巴西圣保罗州流行区和非流行区蝙蝠体内婴儿利什曼原虫和亚马逊利什曼原虫的检测。

目的：利什曼病是巴西几个地区的一种地方病，巴西是一个热带国家，其特定的环境条件有助于白蛉病媒的发展。本研究旨在检测来自圣保罗州17个市的自然感染蝙蝠的利什曼原虫种类。方法与结果：对203只蝙蝠的脾脏和肝脏标本进行实时荧光定量PCR分析，常规荧光定量PCR证实，并进行基因测序。6.4%的蝙蝠通过实时PCR扩增利什曼原虫DNA， 3.4%的蝙蝠通过常规PCR扩增利什曼原虫DNA并进行测序。阳性样品被鉴定并纳入GenBank。在molossus M.、nigricans M.和glaucinus E.蝙蝠中确认利什曼原虫种。鉴定出亚马逊利什曼原虫（Leishmania）和查加西利什曼原虫（Leishmania）。这是在研究地区首次在蝙蝠中发现利什曼原虫。所有阳性蝙蝠都来自城市地区。食虫蝙蝠在统计上更为积极。我们的序列与该地区人类分离物和白蛉的序列相似。结论：这一结果指出蝙蝠是巴西利什曼原虫可能的重要宿主，并指导该国卫生当局在流行区开展流行病学监测、控制和预防行动。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zoonoses and Public Health 医学-传染病学

CiteScore

5.30

自引率

4.20%

发文量

115

审稿时长

6-12 weeks

期刊介绍： Zoonoses and Public Health brings together veterinary and human health researchers and policy-makers by providing a venue for publishing integrated and global approaches to zoonoses and public health. The Editors will consider papers that focus on timely collaborative and multi-disciplinary research in zoonoses and public health. This journal provides rapid publication of original papers, reviews, and potential discussion papers embracing this collaborative spirit. Papers should advance the scientific knowledge of the sources, transmission, prevention and control of zoonoses and be authored by scientists with expertise in areas such as microbiology, virology, parasitology and epidemiology. Articles that incorporate recent data into new methods, applications, or approaches (e.g. statistical modeling) which enhance public health are strongly encouraged.