Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2024-12-06 DOI:10.1016/j.resmic.2024.104263

Ana L Wevar Oller, Gonzalo Torres Tejerizo, Paola P Pereira, Romina Del Pilar Pramparo, Elizabeth Agostini

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引用次数: 0

Abstract

Pseudomonas sp. AW4 is a highly arsenic (As) resistant bacterium with plant growth promoting properties, originally isolated from the soybean (Glycine max L.) rhizosphere. In order to safely use this isolate in diverse bioformulations, its characterization needs to be completed and a reliable identification must be provided. In the present work, we analyzed the morpho-physiological, biochemical and genomic characteristics of Pseudomonas sp. AW4. Identification of the isolate varied according to the parameters analyzed, mainly biochemical and physiological tests or individual genes and phylogenetic analyses. In this regard, we performed massive sequencing of its genome, in order to consistently complete its characterization and identification. Pseudomonas sp. AW4 formed a monophyletic clade with P. urmiensis SWRI10, presenting 3.08 % of unique genes against this reference isolate. More than 70 % of AW4 genes were also shared with P. oryziphila strain 1257 NZ and with P. reidholzensis strain CCOS 865. The search for genes related to As resistance evidenced the presence of the operon arsHRBC. Taken together, results of the present work allow identification of this bacterium as Pseudomonas urmiensis AW4 and open up a number of opportunities to study this strain and understand the mechanisms of arsenic resistance and plant growth promotion.

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大豆（Glycine max L.）根际抗砷促进植物生长细菌Pseudomonas sp. AW4的鉴定与鉴定

Pseudomonas sp. AW4是一种具有促进植物生长特性的高砷抗性细菌，最初从大豆（Glycine max L.）根际分离得到。为了在不同的生物制剂中安全地使用该分离物，需要完成其表征并提供可靠的鉴定。本文对假单胞菌AW4的形态、生理、生化和基因组特征进行了分析。分离物的鉴定根据分析的参数不同而不同，主要是生化和生理试验或个体基因和系统发育分析。为此，我们对其进行了大量的基因组测序，以一致地完成其表征和鉴定。假单胞菌sp. AW4与P. urmiensis SWRI10形成了一个单系分支，与该参考分离物具有3.08%的独特基因。oryziphila菌株1257 NZ和P. reidholzensis菌株CCOS 865共有70%以上的AW4基因。对As抗性相关基因的研究证实了操纵子arsHRBC的存在。综上所述，本研究结果为鉴定该细菌为乌尔米假单胞菌AW4提供了机会，并为研究该菌株以及了解其抗砷和促进植物生长的机制开辟了许多机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.