{"title":"From intercropping to monocropping: The effects of Pseudomonas strain to facilitate nutrient efficiency in peanut and soil.","authors":"Tianqi Wang, Kunguang Wang, Nanqi Wang, Dongming Cui, Shiqin Li, Qiaofang Lu, Yuanmei Zuo","doi":"10.1016/j.plaphy.2024.109378","DOIUrl":null,"url":null,"abstract":"<p><p>As an oilseed crop, the yield and quality of peanuts are severely constrained by nutrient deficiencies, particularly in calcareous soils in northern China. Maize-peanut intercropping is an effective strategy to enhance mineral nutrient efficiency in peanuts via plant-microbe interaction, but the underlying mechanisms remain elusive. Here, we conducted experiments using a Pseudomonas strain (Pse.IP6) with diverse beneficial characteristics, which was isolated from the rhizosphere of intercropped peanuts. Additionally, Pse.IP6 exhibits high phylogenetic similarity with the Amplicon Sequence Variants 48 (ASV48) which belongs to Pseudomonas and is positively correlated with Fe in plants and soil in intercropping. To confirm the plant growth-promoting potential of Pse.IP6 and its role in intercropping advantage, we constructed pot experiments. Results revealed that Pse.IP6 promoted shoot growth and root development, as well significantly enhanced SPAD value, net photosynthetic rate, stomatal conductance, and transpiration rate of peanut leaves. Moreover, the application of Pse.IP6 resulted in a notable accumulation of nitrogen (N), phosphorus (P), and potassium (K) in shoot and active iron (Fe) in leaves, accompanied by an increased K-N ratio. The primary reason for the nutrient promotion is the enhancement of the bioavailability of nitrate, ammonium, P, K, and Fe in the rhizosphere. Collectively, our findings demonstrate that Pse.IP6, enriched in intercropping peanut, is a plant growth-promoting bacteria, represented by transferring the intercropping advantage on nutrients activation to monocropping peanuts. Our results offer insights into plant-rhizobacteria interaction mechanisms and therefore provide a rhizobacteria-based pathway to improve nutrient efficiency and productivity of crops.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109378"},"PeriodicalIF":6.1000,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.plaphy.2024.109378","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
As an oilseed crop, the yield and quality of peanuts are severely constrained by nutrient deficiencies, particularly in calcareous soils in northern China. Maize-peanut intercropping is an effective strategy to enhance mineral nutrient efficiency in peanuts via plant-microbe interaction, but the underlying mechanisms remain elusive. Here, we conducted experiments using a Pseudomonas strain (Pse.IP6) with diverse beneficial characteristics, which was isolated from the rhizosphere of intercropped peanuts. Additionally, Pse.IP6 exhibits high phylogenetic similarity with the Amplicon Sequence Variants 48 (ASV48) which belongs to Pseudomonas and is positively correlated with Fe in plants and soil in intercropping. To confirm the plant growth-promoting potential of Pse.IP6 and its role in intercropping advantage, we constructed pot experiments. Results revealed that Pse.IP6 promoted shoot growth and root development, as well significantly enhanced SPAD value, net photosynthetic rate, stomatal conductance, and transpiration rate of peanut leaves. Moreover, the application of Pse.IP6 resulted in a notable accumulation of nitrogen (N), phosphorus (P), and potassium (K) in shoot and active iron (Fe) in leaves, accompanied by an increased K-N ratio. The primary reason for the nutrient promotion is the enhancement of the bioavailability of nitrate, ammonium, P, K, and Fe in the rhizosphere. Collectively, our findings demonstrate that Pse.IP6, enriched in intercropping peanut, is a plant growth-promoting bacteria, represented by transferring the intercropping advantage on nutrients activation to monocropping peanuts. Our results offer insights into plant-rhizobacteria interaction mechanisms and therefore provide a rhizobacteria-based pathway to improve nutrient efficiency and productivity of crops.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.