The application of different biotechnologies in detecting the changes in MAM and their classic discoveries.

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-12-06 DOI:10.1016/j.ab.2024.115744

Shuangshuang Zhang, Yingchun Shao, Mengzhu Su, Yuerong Hao, Yang Yuan, Dongming Xing

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Abstract

Mitochondria-associated membrane (MAM) has been studied as a novel target for explaining the mechanisms underlying the changes in cellular function and the process of multiple diseases. This structure is a complex of proteins, it tethers mitochondria to the endoplasmic/sarcoplasmic reticulum (ER/SR) and mediates the crosstalk of ions, lipids and metabolites between the two organelles. Different component proteins play distinctive ways in influencing the structure of MAM or the cellular signal transduction. Mitochondria and ER are the hubs of cellular bioenergetics and protein homeostasis respectively, MAM was supposed to play both physiological and pathological roles in regulating the function of either the two organelles and cells. The mitochondria-associated membrane is a highly dynamic structure and could be disrupted or remodelled within several minutes. Up to now, not all component proteins of the MAM complex have been revealed. Several biochemical and imaging approaches have been used to measure the changes in the structure or the number of MAMs, but they come with their issues. For distinct research aims, particular techniques were used based on their applicabilities, the research platforms and technical defects. This review briefly summarized the current biotechnologies for detecting MAM, and analyzed their limitations, aiming to assist further research in selecting appropriate methods based on actual situations.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.