Probiotic-Based Mineralized Living Materials to Produce Antimicrobial Yogurts.

IF 10 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Gloria B Ramírez-Rodríguez, Laura Sabio, Laura Cerezo-Collado, Víctor Garcés, Jose M Domínguez-Vera, José M Delgado-López
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引用次数: 0

Abstract

Mineralization of living cells represents an evolutionary adaptation that enhances cellular resilience to physicochemical stress. Inspired by this strategy, we have here developed hybrid living materials (HLMs), incorporating probiotics into mineralized collagen 3D matrices, with the aim of protecting and promoting the successful oral delivery of the bacteria. Collagen fibrils are simultaneously self-assembled and mineralized in the presence of the probiotics (Lactobacillus acidophilus, La, was used as model), resulting in the integration of the probiotics into the hybrid matrix (i.e., bulk encapsulation). During this process, probiotics are also coated with a nanofilm of apatite mineral (single-cell encapsulation), which provides them with extra protection and reinforces their viability and activity. In fact, the resulting HLM is metabolically active, and maintain the capacity to ferment milk into yogurt with antibacterial activity against the two major foodborne pathogens Pseudomonas aeruginosa (Pa) and Staphylococcus aureus (Sa). Interestingly, the HLM provides probiotics an additional protection in the gastrointestinal environment (i.e., simulated gastric fluid), which is of special interest for healthcare materials for oral administration. The results pave the way for the creation of innovative healthcare materials with enhanced functionalities and the potential to produce probiotic foods with notable antimicrobial properties.

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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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