Visualization of soil aggregate structures provides insights into their formation mechanisms induced by litter inputs

IF 9.8 1区 农林科学 Q1 SOIL SCIENCE
Milda Pucetaite , Per Persson , Julia Parker , Ulf Johansson , Edith C. Hammer
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引用次数: 0

Abstract

Soil aggregation is a dynamic process influenced by physical, chemical and biological factors; however, their individual and combined effect on the formation and turnover of aggregates is not well understood. The aim of this study was to examine incorporation of fresh litter inputs of different physicochemical properties including their carbon-to-nitrogen (C/N) ratio – maize (C/N = 12) and straw (C/N = 103) - into aggregates, de novo formed from mineral soil with or without the presence of microbiota. Using rare-earth element oxides, we labelled structures formed during a four-week incubation with a single litter type and traced their incorporation into newly formed aggregates after mixing them together and incubating for a subsequent seven-week period. To visualize them, we used synchrotron-based X-ray fluorescence microspectroscopy, which allowed us to demonstrate that presence of the plant-derived particulate organic matter was the key factor for the aggregate formation. Within the timescale of the experiment, neither microbial abundance nor the community composition had any significant effect. However, the relative increase in straw-associated soil in aggregates larger than 250 μm provided support for our hypothesis regarding impact of carbon-rich organic matter on macroaggregation, likely via promotion of fungal growth and hyphal enmeshing. Phospholipid fatty acid analysis further confirmed relatively higher abundance of fungi in macroaggregates in straw-containing soil. All in all, our study provides insights into the initial stages of aggregate formation following litter additions and development of associated microbial community. The spatial analysis enabled by the X-ray fluorescence microspectroscopy enabled visualization of internal aggregate structures, shedding light on the processes involved, which is not possible with bulk analysis alone.
土壤团聚体结构的可视化提供了对凋落物输入诱导其形成机制的见解
土壤团聚是一个受物理、化学和生物因素影响的动态过程;然而,它们对团聚体形成和周转的单独和联合作用尚不清楚。本研究的目的是研究不同物理化学性质的新鲜凋落物输入,包括它们的碳氮比(C/N)——玉米(C/N = 12)和秸秆(C/N = 103)——在有或没有微生物群存在的矿物土壤中重新形成的团聚体。使用稀土元素氧化物,我们标记了在单一凋落物类型的4周孵育期间形成的结构,并在将它们混合在一起并在随后的7周孵育后追踪它们融入新形成的聚集体的情况。为了可视化它们,我们使用了基于同步加速器的x射线荧光显微光谱,这使我们能够证明植物来源的颗粒有机物质的存在是聚集体形成的关键因素。在实验时间尺度内,微生物丰度和群落组成均无显著影响。然而,秸秆相关土壤在大于250 μm团聚体中的相对增加,支持了我们关于富碳有机质对大团聚体影响的假设,可能是通过促进真菌生长和菌丝缠绕来实现的。磷脂脂肪酸分析进一步证实了含秸秆土壤大团聚体中真菌的丰度较高。总而言之,我们的研究提供了对凋落物添加和相关微生物群落发展后聚集体形成的初始阶段的见解。由x射线荧光微光谱学实现的空间分析使内部聚集体结构可视化,揭示了所涉及的过程,这是单独进行体分析无法实现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Soil Biology & Biochemistry
Soil Biology & Biochemistry 农林科学-土壤科学
CiteScore
16.90
自引率
9.30%
发文量
312
审稿时长
49 days
期刊介绍: Soil Biology & Biochemistry publishes original research articles of international significance focusing on biological processes in soil and their applications to soil and environmental quality. Major topics include the ecology and biochemical processes of soil organisms, their effects on the environment, and interactions with plants. The journal also welcomes state-of-the-art reviews and discussions on contemporary research in soil biology and biochemistry.
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