The use of sex-sorted semen in horses

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES
JC Samper , JC Hernandez Aviles , LF Ramirez-Agamez , CC Love , C Gonzalez-Marin , P Fleury , P Dini , A De La Fuente , R Foss , FL Campos , PJ Ross
{"title":"The use of sex-sorted semen in horses","authors":"JC Samper ,&nbsp;JC Hernandez Aviles ,&nbsp;LF Ramirez-Agamez ,&nbsp;CC Love ,&nbsp;C Gonzalez-Marin ,&nbsp;P Fleury ,&nbsp;P Dini ,&nbsp;A De La Fuente ,&nbsp;R Foss ,&nbsp;FL Campos ,&nbsp;PJ Ross","doi":"10.1016/j.jevs.2024.105251","DOIUrl":null,"url":null,"abstract":"<div><div>The application of sex-sorted semen in horses has historically lagged the bovine industry due to differences in the reproductive physiology and grater variability in equine semen quality. Recent advancements, including SuperGen™ sorters and Ultraplus™ semen processing methods developed by STgenetics® (Navasota, Texas), have significantly improved the efficiency of the sex-sorting process and reduced the sperm damage previously reported. To facilitate the broader use sex-sorted semen in the equine industry, it is essential to address challenges such as shipping cooled semen to a central sorting facility, minimizing oxidative stress and DNA fragmentation, and developing effective methods for short-term cooling or long-term cryopreservation of the sex-sorted sperm. In this study, we evaluated the feasibility of shipping cooled (8 °C) equine semen to a central facility for sorting and assessed the quality of sperm post-sorting and preservation. Results from the improved process showed enhancements in sperm motility, morphology, viability and DNA fragmentation following sex-sorting. Although there was a slight, consistent increase in lipid peroxidation of sex-sorted sperm, the quality of sperm over the first 24 h post-sorting was comparable to that of conventional (non-sorted) cool-shipped semen. Fertility rates with fresh semen did not differ between conventional and sex-sorted sperm. When using frozen-thawed sex-sorted sperm for intracytoplasmic sperm injection (ICSI), consistent blastocyst production was achieved, albeit at slightly lower rates than those observed with conventional semen for the same stallions. Stallion-specific variability may account for some of these differences. Overall, sex-sorted semen used for ICSI resulted in a higher number of embryos of the desired sex compared to those obtained using conventional semen.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"145 ","pages":"Article 105251"},"PeriodicalIF":1.3000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Equine Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0737080624002570","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

The application of sex-sorted semen in horses has historically lagged the bovine industry due to differences in the reproductive physiology and grater variability in equine semen quality. Recent advancements, including SuperGen™ sorters and Ultraplus™ semen processing methods developed by STgenetics® (Navasota, Texas), have significantly improved the efficiency of the sex-sorting process and reduced the sperm damage previously reported. To facilitate the broader use sex-sorted semen in the equine industry, it is essential to address challenges such as shipping cooled semen to a central sorting facility, minimizing oxidative stress and DNA fragmentation, and developing effective methods for short-term cooling or long-term cryopreservation of the sex-sorted sperm. In this study, we evaluated the feasibility of shipping cooled (8 °C) equine semen to a central facility for sorting and assessed the quality of sperm post-sorting and preservation. Results from the improved process showed enhancements in sperm motility, morphology, viability and DNA fragmentation following sex-sorting. Although there was a slight, consistent increase in lipid peroxidation of sex-sorted sperm, the quality of sperm over the first 24 h post-sorting was comparable to that of conventional (non-sorted) cool-shipped semen. Fertility rates with fresh semen did not differ between conventional and sex-sorted sperm. When using frozen-thawed sex-sorted sperm for intracytoplasmic sperm injection (ICSI), consistent blastocyst production was achieved, albeit at slightly lower rates than those observed with conventional semen for the same stallions. Stallion-specific variability may account for some of these differences. Overall, sex-sorted semen used for ICSI resulted in a higher number of embryos of the desired sex compared to those obtained using conventional semen.
在马身上使用按性别分类的精液。
由于生殖生理的差异和马精液质量的更大变异性,马精液的性别分类应用在历史上落后于牛工业。最近的进展,包括由STgenetics®(Navasota, Texas)开发的SuperGen™分选器和Ultraplus™精液处理方法,显著提高了性别分选过程的效率,并减少了先前报道的精子损伤。为了促进性别分类精液在马行业的广泛应用,必须解决一些挑战,如将冷却的精液运送到中央分类设施,最大限度地减少氧化应激和DNA断裂,以及开发有效的短期冷却或长期冷冻保存性别分类精子的方法。在这项研究中,我们评估了将冷却(8°C)的马精液运送到中心设施进行分选的可行性,并评估了精子分选和保存后的质量。结果表明,改进后的过程中,精子活力、形态、活力和DNA片段在性别分类后得到了增强。尽管性别分类精子的脂质过氧化有轻微的持续增加,但在分类后的前24小时内,精子的质量与传统(未分类)冷运精液的质量相当。新鲜精液的生育率在传统精子和按性别分类的精子之间没有差异。当使用冷冻解冻的性别分类精子进行卵胞浆内单精子注射(ICSI)时,可以实现一致的囊胚产生,尽管其产生率略低于对同一种马使用常规精液观察到的囊胚产生率。种马特有的变异可以解释其中的一些差异。总的来说,与使用传统精液获得的胚胎相比,用于ICSI的性别分类精液产生了更多的所需性别的胚胎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Equine Veterinary Science
Journal of Equine Veterinary Science 农林科学-兽医学
CiteScore
2.70
自引率
7.70%
发文量
249
审稿时长
77 days
期刊介绍: Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信