TRPS1 Immunohistochemistry in Salivary Gland Neoplasms: Analysis on Cytology Cell Blocks and Surgical Follow-Up Correlation

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Diagnostic Cytopathology Pub Date : 2024-12-07 DOI:10.1002/dc.25428

Saroja Devi Geetha, Amr Ali, Silvat Sheikh-Fayyaz, Cecilia Gimenez, Karen Chau, Kasturi Das, Oana C. Rosca

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引用次数: 0

Abstract

Introduction

In this study we aim to analyze the TRPS1 immunostaining of salivary gland tumors (SGT) on cytology cell blocks and compare the staining pattern on subsequent surgical resections.

Methods

Malignant SGTs, oncocytomas and basal cell adenomas diagnosed on fine needle aspiration were retrieved from 2019 to 2021 database. Cases with surgical follow-up were selected. TRPS1 staining was performed on cytology cell blocks and surgical specimens. Scoring was interpreted by two pathologists independently.

Results

Our cohort comprised of 58 cases: 45 malignant and 13 benign. TRPS1 scoring was interpreted for 44 cytology and 51 surgical cases. 14 cytology cases lacked tumor cells on deeper levels. For 7 cases, surgical blocks were not retrievable. TRPS1 positivity for cytology and surgical cases were 52% and 47% respectively. In the malignant cohort, TRPS1 was positive in 21/32 (66%) cytology cases and 21/43 (56%) surgical cases. All cases of basal cell adenocarcinoma, carcinoma ex pleomorphic adenoma and salivary duct carcinoma were TRPS1 positive on cytology. All cases of adenoid cystic carcinoma and acinic cell carcinoma were TRPS1 positive on surgical resections. In the benign cohort, TRPS1 was positive in 2/12 (17%) cytology cases; however, none of the surgically resected benign cases showed reactivity (0/8). Cytology-surgical correlation of TRPS1 staining was done in 37 cases. We found 21 concordant and 16 discordant cases. Discordance was highest in mucoepidermoid carcinoma.

Conclusion

TRPS1 is not an entirely specific marker for breast carcinoma. TRPS1 positivity was noted in a substantial number of salivary gland malignant neoplasms. Cases demonstrating discordance in TRPS1 staining pattern on cytology-surgical correlation warrant further exploration.

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唾液腺肿瘤TRPS1免疫组化：细胞学、细胞阻滞和手术随访相关性分析。

在本研究中，我们旨在分析涎腺肿瘤（SGT）细胞学细胞块的TRPS1免疫染色，并比较随后手术切除的染色模式。方法：检索2019 - 2021年数据库中经细针穿刺诊断的恶性sgt、癌细胞瘤和基底细胞腺瘤。选择手术随访的病例。对细胞学细胞块和手术标本进行TRPS1染色。评分由两名病理学家独立解读。结果：我们的队列包括58例：45例恶性，13例良性。对44例细胞学和51例外科病例进行TRPS1评分。14例细胞学检查显示深层肿瘤细胞缺失。7例手术块无法取出。细胞学和外科病例中TRPS1阳性分别为52%和47%。在恶性队列中，21/32（66%）细胞学病例和21/43（56%）外科病例中TRPS1阳性。基底细胞腺癌、癌前多形性腺瘤和涎腺管癌的细胞学检查均为TRPS1阳性。腺样囊性癌和腺泡细胞癌均为TRPS1阳性。在良性队列中，2/12（17%）细胞学病例TRPS1阳性；然而，手术切除的良性病例均未出现反应性（0/8）。37例患者行TRPS1染色细胞学-手术相关性检查。我们发现21例一致，16例不一致。黏液表皮样癌的不一致性最高。结论：TRPS1不是乳腺癌的完全特异性标志物。在大量涎腺恶性肿瘤中可见TRPS1阳性。细胞学-外科相关性显示TRPS1染色模式不一致的病例值得进一步探讨。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic Cytopathology 医学-病理学

CiteScore

2.60

自引率

7.70%

发文量

163

审稿时长

3-6 weeks

期刊介绍： Diagnostic Cytopathology is intended to provide a forum for the exchange of information in the field of cytopathology, with special emphasis on the practical, clinical aspects of the discipline. The editors invite original scientific articles, as well as special review articles, feature articles, and letters to the editor, from laboratory professionals engaged in the practice of cytopathology. Manuscripts are accepted for publication on the basis of scientific merit, practical significance, and suitability for publication in a journal dedicated to this discipline. Original articles can be considered only with the understanding that they have never been published before and that they have not been submitted for simultaneous review to another publication.