Supplementation of Epimedium polysaccharide (EPS) improves goat semen characteristics following cryopreservation.

IF 2.2 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Reproduction Science Pub Date : 2025-01-01 Epub Date: 2024-11-29 DOI:10.1016/j.anireprosci.2024.107654
Yu Li, Hui Wang, Zhangtao Hu, Guoyu Zhang, Fei Wen, Ming Xian, Songmao Guo, Guangzhi Zhang, Xing Zhang, Jianhong Hu
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引用次数: 0

Abstract

Cryopreservation facilitates long-term semen storage and enables the exchange of genetic material among elite livestock over extensive distances. A decrease in sperm quality is an unavoidable outcome of the cryopreservation process. Prior research has established that incorporating cryoprotectants into the diluent can mitigate freeze-induced damage and enhance sperm quality. This study aims to assess the impact of Epimedium polysaccharide (EPS) on the cryopreservation of goat semen. Samples were obtained from six healthy goats following an initial examination. One portion of the semen was diluted with a base solution containing EPS for treatment purposes, whereas another was diluted without EPS, serving as the control. Results indicated that varying concentrations (1, 2, 3, 4, 5 mg/mL) of EPS in the diluent enhanced both physiological characteristics and antioxidant enzyme activities in cryopreserved goat sperm. Further analysis showed that the 3 mg/mL EPS concentration significantly improved sperm motility (52.10 %), plasma membrane integrity (57.01 %), tail plasma membrane integrity (52.37 %), acrosome integrity rate (52.45 %), and antioxidant enzyme activities relative to other groups (P < 0.05). Additionally, the inclusion of 3 mg/mL EPS substantially improved the sperm's fertilization capability. In conclusion, our experiments confirm that EPS supplementation significantly enhances sperm quality post-freezing, with 3 mg/mL identified as the optimal concentration.

添加淫羊藿多糖(EPS)可改善山羊精液冷冻保存后的性状。
低温保存有利于精液的长期储存,并使遗传物质在优质牲畜之间进行远距离交换。精子质量下降是冷冻保存过程中不可避免的结果。先前的研究已经证实,在稀释剂中加入冷冻保护剂可以减轻冷冻引起的损伤,提高精子质量。本研究旨在探讨淫羊藿多糖(EPS)对山羊精液冷冻保存的影响。在初步检查后,从6只健康山羊身上获得了样本。一部分精液用含有EPS的基础溶液稀释用于治疗目的,而另一部分精液不含EPS稀释作为对照。结果表明,不同浓度(1、2、3、4、5 mg/mL)的EPS对山羊精子的生理特性和抗氧化酶活性均有促进作用。进一步分析表明,3 mg/mL EPS浓度较其他组显著提高了精子活力(52.10 %)、质膜完整性(57.01 %)、尾质膜完整性(52.37 %)、顶体完整性(52.45 %)和抗氧化酶活性(P < 0.05)
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来源期刊
Animal Reproduction Science
Animal Reproduction Science 农林科学-奶制品与动物科学
CiteScore
4.50
自引率
9.10%
发文量
136
审稿时长
54 days
期刊介绍: Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.
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