{"title":"Recent trends in the separation and analysis of chitooligomers.","authors":"Lucie Petrásková, Pavla Bojarová","doi":"10.1016/j.carres.2024.109337","DOIUrl":null,"url":null,"abstract":"<p><p>Chitosan is a widely used linear biopolymer composed mainly of glucosamine and to a lesser extent of N-acetylglucosamine units. Many biological activities of chitosan are attributed to its shorter oligomeric chains, which consist of chitosan prepared either by enzyme activity (lysozyme, bacterial chitinase) or chemically by acid-catalyzed hydrolysis (e.g. in the stomach). However, these processes always result in a mixture of shorter chitooligosaccharides with varying degrees of acetylation whereas for relevant results of biological studies it is necessary to work with a precisely defined material. In this review, we provide an overview and comparison of analytical methods leading to the determination of the degree of polymerization (DP), the degree of acetylation (DA), the fraction of acetylation (FA) and the acetylation patterns (PA) of chitooligosaccharide chains and of the current state of knowledge on chitooligosaccharide separation. This review aims to present the most promising routes to well-defined low molecular weight chitosan with low dispersity.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"548 ","pages":"109337"},"PeriodicalIF":2.4000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.carres.2024.109337","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/28 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Chitosan is a widely used linear biopolymer composed mainly of glucosamine and to a lesser extent of N-acetylglucosamine units. Many biological activities of chitosan are attributed to its shorter oligomeric chains, which consist of chitosan prepared either by enzyme activity (lysozyme, bacterial chitinase) or chemically by acid-catalyzed hydrolysis (e.g. in the stomach). However, these processes always result in a mixture of shorter chitooligosaccharides with varying degrees of acetylation whereas for relevant results of biological studies it is necessary to work with a precisely defined material. In this review, we provide an overview and comparison of analytical methods leading to the determination of the degree of polymerization (DP), the degree of acetylation (DA), the fraction of acetylation (FA) and the acetylation patterns (PA) of chitooligosaccharide chains and of the current state of knowledge on chitooligosaccharide separation. This review aims to present the most promising routes to well-defined low molecular weight chitosan with low dispersity.
期刊介绍：
Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects.
Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence.
Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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