Magnetic assembly of microwires on a flexible substrate for minimally invasive electrophysiological recording.

Abstract

Understanding the neural system in the brain requires the detection of signals from the tissue. Microscale electrodes enable high spatiotemporal neural recording, whereas traditional microelectrodes cause material and geometry mismatches between the electrode and the tissue, leading to injury and signal loss during recording. In this study, we propose a fabrication technique that uses magnetic force to facilitate assembly of vertical microscale wire-electrodes on a flexible substrate. Two-channel 15-μm-diameter and 400-μm-length nickel-microwire electrodes on a 5-μm-thick flexible parylene film are designed and fabricated. Impedance characteristics of these electrodes are <500 kΩ at 1 kHz, with output/input signal amplitude ratios of over 90%. In vivo neural recording in mice demonstrates that both local field potentials and action potentials are detected through each wire electrode, confirming the minimal invasiveness during the electrode penetration and through immunohistochemical tissue analysis.