Proteomics for forensic identification of saliva and vomit in a case of alleged rape.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Analytical and Bioanalytical Chemistry Pub Date : 2025-01-01 Epub Date: 2024-12-07 DOI:10.1007/s00216-024-05674-2
Maria Pieri, Francesco Siano, Pascale Basilicata, Angela Simonelli, Francesco Addeo, Gianluca Picariello
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引用次数: 0

Abstract

In crime investigations, the unambiguous identification of biological traces can be decisive for framing the events. In this study, we applied proteomics to analyze scant amounts of biological residues in the context of an alleged rape case, focusing on the detection of traces of vomit. We used high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and two distinct proteomic workflows to identify proteins and possible proteolytic peptides in biological residues from clothing, bedding, and car upholstery from the alleged crime scene. Specifically, a fragment of pillowcase contained a protein pattern indicative of human saliva and a complex panel of peptides resulting from extensive hydrolysis of salivary proteins. The presence of partly digested proteins from bovine meat, wheat, and eggs, along with salivary and gastric enzymes, demonstrated the presence of vomit on the alleged victim's trousers, also providing insights into the recently consumed meal. A drop of cow's milk on the seat of the suspect's car was likely irrelevant to the criminal act. Other fabric samples showed only common contaminants, excluding significant biological traces or food-derived proteins. These findings support the judicial decision regarding consent to sexual intercourse, for which DNA individualization lacks evidentiary power, and establish a reference for annotating saliva and vomit traces in forensic investigations.

在一起强奸案中,用蛋白质组学对唾液和呕吐物进行法医鉴定。
在犯罪调查中,对生物痕迹的明确识别对于确定事件是决定性的。在这项研究中,我们应用蛋白质组学分析了一起强奸案中少量的生物残留物,重点是检测呕吐物的痕迹。我们使用高效液相色谱-串联质谱(HPLC-MS/MS)和两种不同的蛋白质组学工作流程来鉴定来自所谓犯罪现场的衣服、床上用品和汽车内饰的生物残留物中的蛋白质和可能的蛋白水解肽。具体地说,枕套的一个片段含有一种表明人类唾液的蛋白质模式和一组由唾液蛋白质广泛水解产生的复杂肽。从牛肉、小麦和鸡蛋中部分消化的蛋白质,以及唾液和胃酶的存在,证明了所谓受害者裤子上存在呕吐物,也为了解最近吃过的饭提供了线索。在犯罪嫌疑人的汽车座位上的一滴牛奶可能与犯罪行为无关。其他织物样本只显示了常见的污染物,不包括重要的生物痕迹或食物来源的蛋白质。这些发现支持了DNA个体化缺乏证据力的性行为同意的司法判决,并为法医调查中唾液和呕吐痕迹的注释提供了参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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