Production of polyhydroxyalkanoate from new isolated bacteria of Acidovorax diaphorobacter ZCH-15 using orange peel and its underlying metabolic mechanisms.

IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-04 DOI:10.1016/j.biortech.2024.131949
Jinju Hou, Lei Cheng, Shudong Zhang, Xiaotong Zhang, Xilong Zheng, Qiuzhuo Zhang
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Abstract

Polyhydroxyalkanoate (PHA) is considered a sustainable alternative to traditional petroleum-based plastics due to its biodegradability and biocompatibility. In this study, Acidovorax diaphorobacter ZCH-15, an efficient PHA-producing strain, was isolated from activated sludge. Using food waste-derived orange peel as a substrate, the strain initially achieved a PHA concentration of 0.39 g/L. Under optimal fermentation conditions (30℃, pH 8, 2 % inoculum concentration, and 30 g/L carbon source), the PHA concentration increased by 138 % to reach a maximum of 0.93 g/L. Proton nuclear magnetic resonance spectroscopy and gas chromatography analyses identified the PHA composition as poly(3-hydroxybutyrate-co-3-hydroxyvalerate), which exhibited high crystallinity and structural stability. Metabolomic analysis indicated that the tricarboxylic acid cycle and pentose phosphate pathway were involved in producing succinyl-CoA, a precursor required for PHA synthesis. This study demonstrates the potential for cost-effective industrial PHA production while enabling the high-value utilization of food waste.

新分离菌株ZCH-15利用橘子皮生产聚羟基烷酸酯及其代谢机制
聚羟基烷酸酯(PHA)由于其生物可降解性和生物相容性被认为是传统石油基塑料的可持续替代品。本研究从活性污泥中分离出一株高效产pha菌株-酸ovorax diaphorobacter ZCH-15。以食物垃圾衍生的橙皮为底物,菌株最初的PHA浓度为0.39 g/L。在最佳发酵条件下(30℃、pH 8、接种量2 %、碳源30 g/L), PHA浓度提高138 %,最高可达0.93 g/L。质子核磁共振和气相色谱分析鉴定PHA的组成为聚(3-羟基丁酸酯-co-3-羟基戊酸酯),具有较高的结晶度和结构稳定性。代谢组学分析表明,三羧酸循环和戊糖磷酸途径参与了琥珀酰辅酶a的产生,这是合成PHA所需的前体。这项研究表明,在实现食物垃圾高价值利用的同时,PHA工业生产具有成本效益的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bioresource Technology
Bioresource Technology 工程技术-能源与燃料
CiteScore
20.80
自引率
19.30%
发文量
2013
审稿时长
12 days
期刊介绍: Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.
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