{"title":"GhCOMT33D modulates melatonin synthesis, impacting plant response to Cd<sup>2+</sup> in cotton via ROS.","authors":"Menghao Zhang, Xiugui Chen, Ning Wang, Lijun Guan, Lidong Wang, Xiao Chen, Zhining Yang, Yuping Sun, Yapeng Fan, Yuan Meng, Mengyue Liu, Wenhua Chen, Fange Wu, Ruize Song, Shuai Wang, Xuke Lu, Junjuan Wang, Lixue Guo, Lanjie Zhao, Hongyu Nan, Kunpeng Zhang, Keyun Feng, Wuwei Ye","doi":"10.1111/ppl.14647","DOIUrl":null,"url":null,"abstract":"<p><p>Caffeic acid-3-O-methyltransferase (COMT) serves as the final pivotal enzyme in melatonin biosynthesis and plays a crucial role in governing the synthesis of melatonin in plants. This research used bioinformatics to analyze the phylogenetic relationships, gene structure, and promoter cis-acting elements of the upland cotton COMT gene family members, which it identified as the key gene GhCOMT33D to promote melatonin synthesis and responding to Cd<sup>2+</sup> stress. After silencing GhCOMT33D through virus-induced gene silencing (VIGS), cotton seedlings showed less resistance to Cd<sup>2+</sup> stress. Under Cd<sup>2+</sup> stress, the melatonin content in the silenced plants significantly decreased, while ROS, MDA, and proline accumulated in the plant cells. The stomatal aperture of the leaves was reduced, hindering normal photosynthesis, leading to cotton leaves withering and yellowing, and epidermal cells becoming twisted and deformed, with a large number of gaps appearing. The non-silenced plants had a significantly higher melatonin content and were in better condition, providing important evidence for further research on how plant melatonin enhances the Cd<sup>2+</sup> resistance of cotton and its regulatory mechanisms.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 6","pages":"e14647"},"PeriodicalIF":5.4000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.14647","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Caffeic acid-3-O-methyltransferase (COMT) serves as the final pivotal enzyme in melatonin biosynthesis and plays a crucial role in governing the synthesis of melatonin in plants. This research used bioinformatics to analyze the phylogenetic relationships, gene structure, and promoter cis-acting elements of the upland cotton COMT gene family members, which it identified as the key gene GhCOMT33D to promote melatonin synthesis and responding to Cd2+ stress. After silencing GhCOMT33D through virus-induced gene silencing (VIGS), cotton seedlings showed less resistance to Cd2+ stress. Under Cd2+ stress, the melatonin content in the silenced plants significantly decreased, while ROS, MDA, and proline accumulated in the plant cells. The stomatal aperture of the leaves was reduced, hindering normal photosynthesis, leading to cotton leaves withering and yellowing, and epidermal cells becoming twisted and deformed, with a large number of gaps appearing. The non-silenced plants had a significantly higher melatonin content and were in better condition, providing important evidence for further research on how plant melatonin enhances the Cd2+ resistance of cotton and its regulatory mechanisms.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.