Schwann Cell-Mediated M2-Like Macrophage Polarization in Rhabdomyosarcoma.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2024-12-05 DOI:10.1111/odi.15214

Guanying Feng, Yibo Guo, Mingtao Chen, Yu Zhang, Zheqi Liu, Chen Sun, Xin Hu, Chengzhong Lin, Yu Liu, Yue Wu, Hao Wu, Xi Yang, Yang Wang, Jinhai Ye, Jiannan Liu, Xu Wang, Tong Ji, Chenping Zhang

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引用次数: 0

Abstract

Objective: To investigate the cellular components and immunological characteristics of the head and neck rhabdomyosarcoma (RMS) microenvironment.

Methods: We conducted single-cell transcriptomics to analyze the cellular components of the RMS microenvironment. CellChat was utilized for analyzing intercellular interactions. The cancer genome atlas database was used for validation. CIBERSORT was applied for immune infiltration profiling. Functional enrichment analyses were performed using the Kyoto Encyclopedia of Genes and Genomes. Gene set scores were calculated using single-sample gene set enrichment analysis. Subcutaneous allograft models and bulk RNA sequencing were used for validation. Flow cytometry and immunohistochemistry were used to identify M2-like macrophages.

Results: Our findings revealed an extremely low presence of neutrophils in RMS samples compared with normal sample. RMS sample with high Schwann cell infiltration exhibited an increase in M2-like macrophage infiltration. Receptor-ligand pairs, specifically MIF-CD74 and PTN-SDC3, were identified between Schwann cells and M2-like macrophages. In the RMS sample characterized by significant Schwann cell infiltration, M2-like macrophages demonstrate robust expression of axon guidance factors and are enriched in the axon guidance pathway.

Conclusions: Our study provides valuable insights into the microenvironment and immunological characteristics of RMS, offering crucial information for further research and potential therapeutic strategies.

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.