Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C-reactive protein for early detection of malignant tumors in dogs

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2024-12-06 DOI:10.1111/jvim.17266

Yoonhee Kim, Wonhee Lee, Byunggak Kim, Bonghwan Jang, Kyung-Rok Yu, Hong Sook Kim, Minok Ryu, Yein Oh, Kyoungwon Seo, Chul Park, Ulsoo Choi, Dongha Bhang, Hwayoung Youn

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引用次数: 0

Abstract

Background

Although the importance of early diagnosis of malignant tumors is a major concern, an easily accessible in-house method has not been established.

Objectives

To investigate the most optimal model by combining thymidine kinase 1 (TK1) autoantibody, extracellular protein kinase A (ECPKA) autoantibody, TK1 antigen, C-reactive protein (CRP), age, breed, and sex.

Animals

Serum samples from 1702 dogs were collected from local animal hospitals and referral animal medical centers in Korea.

Methods

TK1 autoantibody, ECPKA autoantibody, and CRP were measured using LFIA methods in serum samples of dog to design a new neoplastic index (NI) for early detection of malignant tumors in dogs.

Results

AUC of TK1 autoantibody model with TK1 antigen, CRP, age, and breed in multiple logistic regression analysis was 0.966 (TK1 autoantibody, P = .0005; TK1 antigen, P = .0003), and when the cutoff value was 0.417, the specificity was 87.1%, and sensitivity was 96.4%. Regression coefficients were 24.4, 20.5, 24.8, 10.6, respectively in TK1 autoantibody, TK1 antigen, CRP, and age, and the effect by breed (regression coefficient 2.1, 3.0) was the lowest. The same multiple logistic regression analysis on dogs with lymphoma, and AUC of TK1 autoantibody model was 0.981 (all P < .0001, TK1 antigen P = .09), when the cutoff value was 0.352, the specificity was 92.9%, and sensitivity was 93.7%.

Conclusions and Clinical Importance

The NI including TK1 autoantibody could be useful in the screening test for both lymphoma and other tumors.

Abstract Image

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胸苷激酶1自身抗体、胸苷激酶抗原、细胞外蛋白激酶A自身抗体和c反应蛋白在犬恶性肿瘤早期检测中的应用

背景：虽然恶性肿瘤的早期诊断的重要性是一个主要的关注，一个容易获得的内部方法尚未建立。目的：结合胸苷激酶1 （TK1）自身抗体、细胞外蛋白激酶A （ECPKA）自身抗体、TK1抗原、c反应蛋白（CRP）、年龄、品种、性别，探讨最优模型。动物：从韩国地方动物医院和转诊动物医疗中心收集了1702只狗的血清样本。方法：采用LFIA法检测犬血清样本中的TK1自身抗体、ECPKA自身抗体和CRP，设计一种新的肿瘤指数（NI），用于犬恶性肿瘤的早期检测。结果：经多元logistic回归分析，TK1自身抗体模型与TK1抗原、CRP、年龄、品种的AUC为0.966 (TK1自身抗体，P = 0.0005；TK1抗原，P = 0.0003)，截止值为0.417时，特异性为87.1%，敏感性为96.4%。TK1自身抗体、TK1抗原、CRP和年龄的回归系数分别为24.4、20.5、24.8、10.6，品种对TK1的影响最小（回归系数分别为2.1、3.0）。对淋巴瘤犬进行相同的多元logistic回归分析，TK1自身抗体模型的AUC为0.981 （P均为P）。结论及临床意义：含TK1自身抗体的NI可用于淋巴瘤及其他肿瘤的筛查试验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.