Molecular identification and subtyping of Cryptosporidium spp. in laboratory mice and rats.

IF 2.3 2区医学 Q2 PARASITOLOGY

Parasite Pub Date : 2024-01-01 Epub Date: 2024-12-04 DOI:10.1051/parasite/2024073

Shanshan Zhou, Xinyu Hu, He Li, Zhongying Yuan, Zhen Li, Aiqin Liu, Yanyan Jiang, Jianping Cao

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Abstract

Cryptosporidium species can infect humans and more than 260 animal species, including 54 rodent species. However, data on the occurrence and genetic characterizations of Cryptosporidium spp. in laboratory rodents are limited. The present study aimed to determine the occurrence rate and genetic characterizations of Cryptosporidium spp. in laboratory mice and rats. We collected 506 fresh combined fecal pellet specimens (457 from mice and 49 from rats) of more than 2,000 laboratory rodents in Heilongjiang Province and Shanghai City, China. Cryptosporidium spp. were identified and subtyped by DNA sequencing of the SSU rRNA and the gp60 genes, respectively. By sequence analysis of the SSU rRNA gene, the occurrence rate of Cryptosporidium spp. was 16.6% (84/506) in combined fecal specimens, with 18.2% (83/457) for mice and 2.0% (1/49) for rats. Cryptosporidium parvum (n = 39), C. tyzzeri (n = 33), and C. parvum + C. tyzzeri (n = 11) were identified in mice. Cryptosporidium parvum was only detected in one rat fecal specimen. At the gp60 locus, 71.4% (60/84) of the Cryptosporidium-positive specimens were successfully amplified, and they all came from mice. We identified five C. parvum subtypes (IIaA14G2R1, IIaA16G2R1, IIaA17G1R1, IIaA17G2R1, and IIaA18G2R1) and two C. tyzzeri subtypes (IXaA6R1 and IXbA8). Based on the identification in laboratory mice of C. parvum subtypes that have been reported previously in humans, the mice infected with this species may threaten human health, especially for people who have contact with the animals and their feces.

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实验小鼠和大鼠隐孢子虫的分子鉴定和分型。

隐孢子虫可以感染人类和260多种动物，其中包括54种啮齿动物。然而，关于隐孢子虫在实验室啮齿动物中的发生和遗传特征的数据是有限的。本研究旨在确定隐孢子虫在实验小鼠和大鼠中的发病率和遗传特征。我们在黑龙江省和上海市采集了2000余只实验啮齿动物的新鲜联合粪便颗粒标本506份（小鼠457份，大鼠49份）。通过对SSU rRNA和gp60基因的DNA测序，分别鉴定了隐孢子虫属，并进行了分型。通过SSU rRNA基因序列分析，隐孢子虫在合并粪便标本中的感染率为16.6%(84/506)，其中小鼠为18.2%(83/457)，大鼠为2.0%（1/49）。在小鼠中鉴定出细小隐孢子虫（39例）、C. tyzzeri（33例）和C. parum + C. tyzzeri（11例）。小隐孢子虫仅在一份大鼠粪便标本中检出。在gp60位点，71.4%（60/84）隐孢子虫阳性标本成功扩增，且全部来自小鼠。鉴定出5种细小弧菌亚型（IIaA14G2R1、IIaA16G2R1、IIaA17G1R1、IIaA17G2R1和IIaA18G2R1）和2种tyzzeri弧菌亚型（IXaA6R1和IXbA8）。根据先前在人类中报告的在实验室小鼠中鉴定出的小孢子虫亚型，感染该物种的小鼠可能威胁人类健康，特别是对与动物及其粪便接触的人。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.