Use of diatomaceous earth to control nymphal American dog ticks, Dermacentor variabilis Say (Acari: Ixodidae): laboratory to simulated field experiments.

Abstract

Diatomaceous earth (DE) recently was shown to rapidly kill ticks. Proof of concept for use of DE to kill the American dog tick, Dermacentor variabilis, the most widely distributed native three-host tick in North America, has not been investigated. Unfed D. variabilis nymphs dipped into DE and incubated at 30 °C and 50 versus 70% relative humidity (RH) had an estimated median survival time of 5 h and 4 h, respectively, with mortality starting within 1 h. There was no difference in survival time at the 50 and 70% RH. In a two-choice assay at 30 °C and 50% RH, ticks walked freely onto DE treated surfaces; the estimated median survival time was 3 h, indicating that DE is not a repellent. There was no difference in locomotor activity between DE-treated nymphs and untreated controls. In simulated-field experiments, pine straw infested with nymphs and then treated with DE at 5.00, 2.50, 1.25, 0.63 and 0.31 g DE/m² resulted in 100% mortality and 81.40% (± 4.13%) mortality at 0.15 g DE/m² after 24 h. Scanning electron microscopy of the dead ticks in the 0.15 g DE/m² treatments showed most of the cuticle surface was devoid of DE except for the hypostome. The rapid time to first death by dipping, the no effect of RH on survival time, the small amounts of mineral on the tick body and no visible scarring on the tick cuticle questions the generally accepted mode of action for DE, that is, cuticle damage and dehydration.