Urine and serum-based ELISA using a recombinant protein and synthetic peptide for the diagnosis of tegumentary leishmaniasis

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-11-27 DOI:10.1016/j.diagmicrobio.2024.116631

Camila S. Freitas , Raquel S.B. Câmara , Daniela P. Lage , Danniele L. Vale , Ana L. Silva , Breno L. Pimenta , Fernanda Ludolf , Nathália C. Galvani , Marcelo M. de Jesus , Bárbara P.N. Assis , Ana T. Chaves , Grasiele S.V. Tavares , Unaí Tupinambás , Miguel A. Chávez-Fumagalli , Vanessa P.M. Pascoal , Marcela T.C. Eller , Manoel O. da Costa Rocha , Myron Christodoulides , Ricardo A. Machado-de-Ávila , Denise U. Gonçalves , Eduardo A.F. Coelho

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Abstract

The diagnosis of tegumentary leishmaniasis (TL) presents problems by the variable sensitivity and specificity of the tests, and the biological samples used are also invasive. Here, ELISA experiments were performed using paired TL patient urine and serum samples in reaction against the recombinant LiHyS protein, a predicted B cell epitope and parasite antigenic extract (SLA). Two hundred and five paired samples were used, which were provided by TL patients, healthy controls and patients with Chagas disease, leprosy, malaria or HIV-infected. An urine-based ELISA showed sensitivity values of 100%, 92.1%, and 82.5%, when rLiHyS, peptide and SLA were used, respectively; and specificity of 100%, 87.6%, and 79.5%, respectively. A serum-based ELISA showed sensitivity values of 100%, 99.3%, and 81.5%, using rLiHyS, peptide and SLA, respectively, and sensitivity of 100%, 96.5%, and 72.2%, respectively. In both cases, rLiHyS presented the better performance to diagnose TL by using patient serum and urine.

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利用重组蛋白和合成肽的尿液和血清酶联免疫吸附试验诊断被膜利什曼病。

囊状利什曼病（TL）的诊断由于检测的敏感性和特异性不同而存在问题，而且所使用的生物样本也是侵入性的。在这里，ELISA实验使用配对的TL患者尿液和血清样本对重组LiHyS蛋白、预测的B细胞表位和寄生虫抗原提取物（SLA）进行反应。使用了225个配对样本，这些样本由TL患者、健康对照者和南美锥虫病、麻风病、疟疾或艾滋病毒感染者提供。当使用rLiHyS、peptide和SLA时，基于尿液的ELISA检测灵敏度分别为100%、92.1%和82.5%；特异性分别为100%、87.6%和79.5%。采用rLiHyS、peptide和SLA，基于血清的ELISA检测灵敏度分别为100%、99.3%和81.5%，灵敏度分别为100%、96.5%和72.2%。在这两种情况下，rLiHyS通过患者血清和尿液诊断TL表现出更好的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.