Molecular design strategy for microorganism discrimination based on keto-salicylaldehyde azine derivatives

IF 10.7 1区 生物学 Q1 BIOPHYSICS
Caihong Yan , Kexin Zhang , Yuhang Zeng , Qiyun Deng , Fan Zhou , Jiayi Li , Hanyi Gao , Ying Tian , Rong Hu , Zhiming Wang , Ben Zhong Tang
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引用次数: 0

Abstract

The rapid and selective identification of microorganisms is of great significance for clinical therapy applications. To develop high performance probes for microbe determination, we systemically constructed series aggregation-induced emission (AIE) luminogens by modulating the structural planarity, the basicity of functional group, the length of linker moiety and the hydrophobicity based on our previous work. The detail structure-property relationship study based on experimental and theoretical observation revealed that: i) the planar skeleton is essential for probe insertion towards the cell wall via van n der Waals' force. ii) the basic function group enable the anchoring on the membrane by binding with acidic biomolecules. iii) the shortened alkyl chain is in favor of the efficient binding of basic groups with microbes and endows the desirable hydrophobicity. Based on the developed probes, the successful detection of the pathogens in clinic samples was achieved in highly sensitive and simple way. This work provides a reliable strategy for designing intelligent luminogens for microorganism discrimination and identification in efficient and sensitive way for in vitro diagnosis applications, especially point-of-care testing (POCT).
基于酮水杨醛嗪衍生物的微生物识别分子设计策略。
微生物的快速、选择性鉴定对临床治疗应用具有重要意义。为了开发高性能的微生物检测探针,我们在前人研究的基础上,系统地通过调节结构平面度、官能团碱度、连接体长度和疏水性,构建了系列聚集诱导发射(AIE)发光物质。基于实验和理论观察的详细结构-性能关系研究表明:1)平面骨架是探针通过范德华力插入细胞壁所必需的;Ii)碱性官能团通过与酸性生物分子结合使其锚定在膜上。Iii)缩短的烷基链有利于碱性基团与微生物的有效结合,并具有理想的疏水性。基于所研制的探针,以高灵敏度和简便的方法成功地检测了临床样品中的病原菌。本研究为设计智能发光源,高效、灵敏地用于体外诊断,特别是即时检测(POCT)提供了可靠的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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