Unveiling cellular mysteries: Advances in BODIPY dyes for subcellular imaging

IF 20.3 1区 化学 Q1 CHEMISTRY, INORGANIC & NUCLEAR
Haroon Ahmad , Sibtain Muhammad , Maria Mazhar , Ahmad Farhan , Muhammad Sohaib Iqbal , Hind Hiria , Chen Yu , Yinghe Zhang , Bing Guo
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Abstract

Fluorescence imaging has become crucial in cell biology, mainly because of its ability to visualize the cells at the molecular level. Subcellular organs including mitochondria, lysosomes, the endoplasmic reticulum, and the Golgi apparatus play a pivotal role in the physiological and pathological processes in living cells. Among the great number of fluorescent trackers developed so far is 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY), which has the following features: high quantum yields, sharp excitation and emission maxima, high stability, and feasibility to tune chemical, physical, and biological properties. Lately, there has been great progress towards enhancing BODIPY dyes that are water-soluble, cell membrane-permeable, and precisely targeted. Herein, this review article will introduce the BODIPY probes for subcellular imaging with application to various regions of living cells or tissues. Furthermore, we describe the process of functionalizing BODIPY probes to enhance their selectivity and preferential localization in organelles. Moreover, the targeting mechanism is explained to give insights into how different cellular microenvironments dictate the nature of moieties to be attached to the BODIPY scaffold. In addition, confocal microscopy and colocalization studies are reported to verify the desired subcellular distribution of the probes. Importantly, in vitro and in vivo studies are reported to ascertain the safety of the probes for clinical uses. We hope this review will stimulate interest among researchers and students and expedite the development of BODIPY chemistry in biomedical applications.
揭示细胞的奥秘:用于亚细胞成像的BODIPY染料的进展
荧光成像在细胞生物学中已经变得至关重要,主要是因为它能够在分子水平上可视化细胞。包括线粒体、溶酶体、内质网和高尔基体在内的亚细胞器官在活细胞的生理和病理过程中起着关键作用。在目前开发的众多荧光跟踪剂中,4,4-二氟-4-硼-3a,4 -二氮-s-茚二烯(BODIPY)具有量子产率高、激发和发射最大值大、稳定性高、化学、物理和生物性质可调等特点。最近,在增强水溶性、细胞膜渗透性和精确靶向性的BODIPY染料方面取得了很大进展。本文将介绍用于亚细胞成像的BODIPY探针,并将其应用于活细胞或组织的各个区域。此外,我们描述了功能化BODIPY探针的过程,以提高它们在细胞器中的选择性和优先定位。此外,研究人员还解释了靶向机制,以深入了解不同的细胞微环境如何决定附着在BODIPY支架上的部分的性质。此外,共聚焦显微镜和共定位研究报告,以验证所需的亚细胞分布的探针。重要的是,体外和体内研究报告,以确定探针的安全性用于临床应用。我们希望这篇综述能引起研究人员和学生的兴趣,并加快BODIPY化学在生物医学应用中的发展。
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来源期刊
Coordination Chemistry Reviews
Coordination Chemistry Reviews 化学-无机化学与核化学
CiteScore
34.30
自引率
5.30%
发文量
457
审稿时长
54 days
期刊介绍: Coordination Chemistry Reviews offers rapid publication of review articles on current and significant topics in coordination chemistry, encompassing organometallic, supramolecular, theoretical, and bioinorganic chemistry. It also covers catalysis, materials chemistry, and metal-organic frameworks from a coordination chemistry perspective. Reviews summarize recent developments or discuss specific techniques, welcoming contributions from both established and emerging researchers. The journal releases special issues on timely subjects, including those featuring contributions from specific regions or conferences. Occasional full-length book articles are also featured. Additionally, special volumes cover annual reviews of main group chemistry, transition metal group chemistry, and organometallic chemistry. These comprehensive reviews are vital resources for those engaged in coordination chemistry, further establishing Coordination Chemistry Reviews as a hub for insightful surveys in inorganic and physical inorganic chemistry.
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