Haroon Ahmad, Sibtain Muhammad, Maria Mazhar, Ahmad Farhan, Muhammad Sohaib Iqbal, Hind Hiria, Chen Yu, Yinghe Zhang, Bing Guo
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引用次数: 0
Abstract
Fluorescence imaging has become crucial in cell biology, mainly because of its ability to visualize the cells at the molecular level. Subcellular organs including mitochondria, lysosomes, the endoplasmic reticulum, and the Golgi apparatus play a pivotal role in the physiological and pathological processes in living cells. Among the great number of fluorescent trackers developed so far is 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY), which has the following features: high quantum yields, sharp excitation and emission maxima, high stability, and feasibility to tune chemical, physical, and biological properties. Lately, there has been great progress towards enhancing BODIPY dyes that are water-soluble, cell membrane-permeable, and precisely targeted. Herein, this review article will introduce the BODIPY probes for subcellular imaging with application to various regions of living cells or tissues. Furthermore, we describe the process of functionalizing BODIPY probes to enhance their selectivity and preferential localization in organelles. Moreover, the targeting mechanism is explained to give insights into how different cellular microenvironments dictate the nature of moieties to be attached to the BODIPY scaffold. In addition, confocal microscopy and colocalization studies are reported to verify the desired subcellular distribution of the probes. Importantly, in vitro and in vivo studies are reported to ascertain the safety of the probes for clinical uses. We hope this review will stimulate interest among researchers and students and expedite the development of BODIPY chemistry in biomedical applications.
期刊介绍:
Coordination Chemistry Reviews offers rapid publication of review articles on current and significant topics in coordination chemistry, encompassing organometallic, supramolecular, theoretical, and bioinorganic chemistry. It also covers catalysis, materials chemistry, and metal-organic frameworks from a coordination chemistry perspective. Reviews summarize recent developments or discuss specific techniques, welcoming contributions from both established and emerging researchers.
The journal releases special issues on timely subjects, including those featuring contributions from specific regions or conferences. Occasional full-length book articles are also featured. Additionally, special volumes cover annual reviews of main group chemistry, transition metal group chemistry, and organometallic chemistry. These comprehensive reviews are vital resources for those engaged in coordination chemistry, further establishing Coordination Chemistry Reviews as a hub for insightful surveys in inorganic and physical inorganic chemistry.