Pinpointing MQTLs and candidate genes related to early maturity in upland cotton through the integration of meta‑analysis, RNA-seq, and VIGS approaches

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-01-01 DOI:10.1016/j.indcrop.2024.120195

Wenmin Yuan , Ying Li , Wenjiao Zhang , Jisheng Ju , Xuefeng Guo , Junning Yang , Hai Lin , Caixiang Wang , Qi Ma , Junji Su

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引用次数: 0

Abstract

Early maturity is one of the primary traits associated with fiber yield, fiber quality and mechanized harvesting in China’s cotton agriculture. Mapping QTLs and mining candidate genes associated with maturity in cotton are critical for molecular improvement efforts that aim to increase early maturity traits in cotton. Integrating a large collection of cotton QTLs is an effective approach for revealing meta-QTLs (MQTLs) to identify candidate genes influencing early maturity in upland cotton. In this research, 1096 original QTLs linked to nine early maturity related traits were identified from 37 independent studies. Additionally, 772 initial QTLs were projected onto a consensus map containing 5051 markers, and 84 MQTLs were identified for traits related to early maturity. Among the MQTLs, 37 regions were verified via SNP loci reported in genome-wide association studies (GWAS) on early maturity traits in cotton. Finally, 118 candidate genes were successfully annotated in five major MQTL regions. Two candidate genes (GhMYOB7 and GhNSRB) linked to early maturity were identified via RNA-seq and qRTPCR experiments. Furthermore, virus-induced gene silencing (VIGS) revealed that GhMYOB7 and GhNSRB are crucial genes that control early flowering in cotton. The results of this study lay a foundation for exploring the genetic mechanisms underlying early maturity traits in upland cotton and provide a theoretical basis for the development of early maturity cotton cultivars.

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通过整合meta分析、RNA-seq和VIGS方法，精确定位陆地棉花早熟相关的mqtl和候选基因

早熟是影响棉花产量、品质和机械化收获的主要性状之一。定位与棉花成熟度相关的qtl和候选基因对于提高棉花早熟性状的分子改良工作至关重要。整合大量棉花qtl是揭示meta- qtl、鉴定陆地棉早熟候选基因的有效方法。在这项研究中，从37个独立研究中鉴定出1096个与9个早熟相关性状相关的原始qtl。此外，772个初始qtl被投射到包含5051个标记的共识图上，其中84个mqtl被鉴定为与早熟相关的性状。其中37个区域通过棉花早熟性状全基因组关联研究（GWAS）中报道的SNP位点进行了验证。最终，118个候选基因在5个主要的MQTL区域中被成功标注。通过RNA-seq和qRTPCR实验鉴定了两个与早熟相关的候选基因（GhMYOB7和GhNSRB）。此外，病毒诱导的基因沉默（VIGS）揭示了GhMYOB7和GhNSRB是控制棉花早花的关键基因。本研究结果为探索陆地棉早熟性状的遗传机制奠定了基础，并为早熟棉品种的培育提供了理论依据。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.