Distinct N-cycling microbial communities contribute to microtopographic variation in soil N2O emissions from denitrification

IF 9.8 1区 农林科学 Q1 SOIL SCIENCE
Alexander H. Krichels, Robert A. Sanford, Joanne C. Chee-Sanford, Lynn Connor, Rachel Van Allen, Angela D. Kent, Wendy H. Yang
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引用次数: 0

Abstract

Climate change is increasing the frequency and intensity of large precipitation events that flood soils and establish anoxic conditions that promote microbial denitrification, a predominant source of atmospheric nitrous oxide (N2O, a strong greenhouse gas). Denitrification may be favored within topographic depressions in otherwise flat fields that are prone to ponding, establishing “hotspots” of N2O emissions. The location of N2O hotspots may also depend on the distribution of soil microbial communities that are responsible for the production and consumption of N2O in soils. Yet, relating soil microbial community composition to N2O emissions remains challenging. To assess how spatial variation in soil microbial communities affects N2O emissions, we measured the community composition of active microorganisms using amplicon-based sequencing of cDNA generated from mRNA transcripts associated with N-cycling processes in response to experimentally flooding and draining soils in the lab. We also used stable isotope tracers to relate microbial communities to process rates. Consistent with the hypothesis that denitrifying microbial communities are not functionally redundant, we found that the diversity of microbial taxa expressing nitrite reduction genes (nirK) and N2O reduction genes (Clade I nosZ) were correlated with denitrifier-derived N2O emissions. Depressional soils had more diverse active N2O consuming communities (assessed using Clade I nosZ) under flooded conditions, limiting net N2O emissions compared to upslope soils. Our results show that depressional soils maintain distinct microbial communities that likely promote higher rates of N2O reduction compared to upslope soils. Soil microtopography can, therefore, select for distinct microbial communities that emit different amount of N2O in response to large precipitation events.
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来源期刊
Soil Biology & Biochemistry
Soil Biology & Biochemistry 农林科学-土壤科学
CiteScore
16.90
自引率
9.30%
发文量
312
审稿时长
49 days
期刊介绍: Soil Biology & Biochemistry publishes original research articles of international significance focusing on biological processes in soil and their applications to soil and environmental quality. Major topics include the ecology and biochemical processes of soil organisms, their effects on the environment, and interactions with plants. The journal also welcomes state-of-the-art reviews and discussions on contemporary research in soil biology and biochemistry.
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