Insight into natural attenuation of tributyl phosphate by indigenous anaerobic microbes in soils: Implication by stable carbon isotope fractionation and microbial community structures

Abstract

Organophosphate esters (OPEs) are widespread in the environment, with high persistence and toxicity. However, the underlying mechanisms of anaerobic microbial degradation of OPEs remain elusive in the field environment. In this study, the natural attenuation mechanisms of tributyl phosphate (TnBP) by indigenous anaerobic microorganisms in soils were investigated by using compound-specific stable isotope analysis (CSIA) and characterization of microbial communities. The results indicated that dibutyl phosphate (DnBP) was the major degradation product of TnBP. Significant carbon isotope fractionation was observed for TnBP during the anaerobic microbial degradation, and the carbon isotope enrichment factor (ε_C) was determined to be -2.71 ± 0.13‰. Unlike aerobic degradation with P-O bond cleavage, C-O bond cleavage was verified as the mode to removal a butyl side chain for TnBP to generate DnBP during the anaerobic microbial degradation. Microbial community analysis indicated that Sphingomonans, Nocardioides and Streptomyces were the important contributors to microbial degradation of TnBP in anoxic soils. TnBP altered microbial metabolic functions in anoxic soils, mainly enhancing the biosynthesis of ansamycins, ketone bodies and amino acids, and flagellar assembly, which promoted microbial degradation of TnBP. This study provided a better method to characterize the chemical bond cleavage mode and effect of OPEs on microbial communities, which was a prerequisite for the bioremediation of OPE pollution in soils.