Women with pelvic organ prolapse and fibulin-5 rs12589592 polymorphism.

Revista da Associacao Medica Brasileira (1992) Pub Date : 2024-12-02 eCollection Date: 2024-01-01 DOI:10.1590/1806-9282.20240687
Fabiana Garcia Reis Maeda, Claudia Cristina Palos, Cesar Eduardo Fernandes, Ricardo Peres do Souto, Emerson de Oliveira
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Abstract

Objective: This study aims to access the frequency of single-nucleotide polymorphism rs12589592 (G>A) of the fibulin-5 gene in a Brazilian population with pelvic organ prolapse.

Methods: This was a case-control study, with menopausal women divided into two groups and classified using the pelvic organ prolapse quantification system: pelvic organ prolapse group: pelvic organ prolapse quantification system stages III and IV and Control group: pelvic organ prolapse quantification system stages I and 0. We collected epidemiologic and baseline health information and performed genotyping of rs12589592 from the fibulin-5 gene using a restriction fragment length polymorphism (polymerase chain reaction-restriction fragment length polymorphism) strategy, based on the distinction of sequences from alleles G and A by the restriction enzyme DdeI. For the pelvic organ prolapse group and control, 111 and 180 women were recruited, respectively.

Results: The rs12589592 (G>A) polymorphism analysis showed 141 GG homozygotes (pelvic organ prolapse group: 53 [47.7%] and Control: 88 [49.2%] p=0.850); and 149 AA+GA: (pelvic organ prolapse group: 58 [52.3%]; Control: 91 [50.8%]; p=0.904). The distribution of genotypes did not follow the Hardy-Weinberg equilibrium conditions.

Conclusion: There was no difference between groups regarding genotypes (rs12589592 G>A) frequency; however, the population characteristics prevent the analysis of the association between the genotype and the occurrence of prolapse.

女性盆腔器官脱垂与纤蛋白-5 rs12589592多态性
目的:本研究旨在了解巴西盆腔器官脱垂人群中纤维蛋白-5基因单核苷酸多态性rs12589592 (G>A)的频率。方法:采用病例对照研究,将绝经期妇女分为两组,采用盆腔脏器脱垂量化系统进行分级:盆腔脏器脱垂组:盆腔脏器脱垂量化系统III期和IV期;对照组:盆腔脏器脱垂量化系统I期和0期。我们收集了流行病学和基线健康信息,并基于限制性内切酶DdeI对等位基因G和a序列的区分,采用限制性内切片段长度多态性(polymerase chain reaction-限制性片段长度多态性)策略对纤蛋白-5基因rs12589592进行了基因分型。盆腔器官脱垂组和对照组分别招募了111名和180名妇女。结果:rs12589592 (G>A)多态性分析显示141个GG纯合子(盆腔器官脱垂组53个[47.7%],对照组88个[49.2%]p=0.850);AA+GA组149例:(盆腔器官脱垂组58例[52.3%];对照组:91人[50.8%];p = 0.904)。基因型分布不符合Hardy-Weinberg平衡条件。结论:基因型(rs12589592 G>A)频率组间无差异;然而,群体特征阻碍了基因型与脱垂发生之间关系的分析。
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