tRNA, yRNA, and rRNA fragment excisions do not involve canonical microRNA biogenesis machinery.

microPublication biology Pub Date : 2024-11-19 eCollection Date: 2024-01-01 DOI:10.17912/micropub.biology.001332
Noel L Godang, Anita D Nguyen, Jeffrey D DeMeis, Sunita S Paudel, Nick J Campbell, Kingston J Barnes, Kahyeon Jeon, Alayla S Roussell, Kimberly A Gregson, Glen M Borchert
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Abstract

The excision of specific tRNA-derived small RNAs (tsRNAs), yRNA-derived small RNAs (ysRNAs) and ribosomal RNA-derived small RNAs (rsRNAs) is now well established. Several reports have suggested many of these fragments function much like traditional microRNAs (miRNAs). That said, whereas the expressions of the majority of appreciably expressed miRNAs in HCT116 colon cancer cells are significantly decreased in individual knockouts (KOs) of DROSHA, DGCR8, XPO5, and DICER, on average, only 3.5% of tsRNA, ysRNA, and rsRNA expressions are impaired. Conversely, tsRNA, ysRNA, and rsRNA expressions are significantly increased in each of these KOs as compared to WT. As such, although DICER has been suggested to be involved with the expression of specific tsRNAs, ysRNAs, and rsRNAs, our study finds no evidence supporting the involvement of any of these canonical miRNA biogenesis enzymes in their expressions.

tRNA, yRNA和rRNA片段切除不涉及标准的microRNA生物发生机制。
特异性trna衍生小rna (tsRNAs)、yrna衍生小rna (ysRNAs)和核糖体rna衍生小rna (rsRNAs)的切除现已得到很好的证实。一些报告表明,许多这些片段的功能与传统的microrna (mirna)非常相似。也就是说,尽管在DROSHA、DGCR8、XPO5和DICER的个体敲除(KOs)中,HCT116结肠癌细胞中大多数明显表达的mirna的表达显著降低,但平均而言,只有3.5%的tsRNA、ysRNA和rsRNA表达受损。相反,与WT相比,tsRNA、ysRNA和rsRNA在这些ko中的表达显著增加。因此,尽管DICER被认为与特定tsRNA、ysRNA和rsRNA的表达有关,但我们的研究没有发现任何证据支持这些典型的miRNA生物发生酶参与它们的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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