High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer.

IF 2 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Revista Panamericana De Salud Publica-pan American Journal of Public Health Pub Date : 2024-12-04 eCollection Date: 2024-01-01 DOI:10.26633/RPSP.2024.128

Gustavo David García Muentes, Fadi Abdul Latif, Claudia Catalina Beltrán Rodríguez, Sebastián Medina González, Juan Carlos Ruiz Cabezas

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引用次数: 0

Abstract

Objective: To determine the distribution of human papillomavirus (HPV) genotypes in invasive cervical cancer samples from Ecuadorian women who attended the Cancer Institute (Sociedad de Lucha Contra el Cáncer - SOLCA).

Methods: Archived formalin-fixed paraffin-embedded (FFPE) cervical cancer tissue samples collected during 2017-2021 were deparaffinized, and nucleic acid extraction and purification was performed using silica columns. The obtained nucleic acids were analyzed using INNO-LiPA® HPV Genotyping Extra II per the manufacturer's specifications. Data were retrieved from records, and HPV genotypes were determined from the FFPE samples.

Results: The study included samples from 190 women diagnosed with invasive cervical cancer, with a median age of 52.78 years. Squamous cell carcinoma accounted for 78.94% of the cases, while 21.05% had adenocarcinoma. Among the 190 samples, 80.53% tested positive for HPV DNA, while 19.47% were negative. The most common genotypes detected were HPV 16 (64.05%), 18 (16.99%), and 58 (6.54%). HPV infection frequency was higher in samples from patients with elementary level education (p < 0.05).

Conclusions: This study provides valuable insights into the distribution of HPV genotypes in invasive cervical cancer samples from Ecuadorian women. The results indicate an elevated presence of HPV 16, HPV 18, and HPV 58, which are vaccine-preventable genotypes.

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厄瓜多尔浸润性宫颈癌妇女中HPV高危可预防基因型的高频率

目的：确定厄瓜多尔妇女在癌症研究所（Sociedad de Lucha Contra el Cáncer - SOLCA）浸润性宫颈癌样本中人乳头瘤病毒（HPV）基因型的分布。方法：对2017-2021年采集的存档的福尔马林固定石蜡包埋（FFPE）宫颈癌组织标本进行脱蜡处理，采用硅胶柱进行核酸提取纯化。根据制造商的规格，使用inin - lipa®HPV基因分型Extra II对获得的核酸进行分析。从记录中检索数据，并从FFPE样本中确定HPV基因型。结果：本研究纳入了190例诊断为浸润性宫颈癌的妇女，中位年龄为52.78岁。鳞状细胞癌占78.94%，腺癌占21.05%。190份样本中，80.53%的人乳头瘤病毒DNA检测阳性，19.47%的人乳头瘤病毒DNA检测阴性。最常见的基因型是HPV 16（64.05%）、18（16.99%）和58（6.54%）。受教育程度较低的人群HPV感染率较高（p < 0.05）。结论：本研究为厄瓜多尔妇女浸润性宫颈癌样本中HPV基因型的分布提供了有价值的见解。结果显示HPV 16， HPV 18和HPV 58的存在升高，这是疫苗可预防的基因型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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