BCP4: A novel antimicrobial peptide with potent efficacy against Bacillus cereus in rice porridge.

Abstract

Bacillus cereus is a common foodborne pathogen that frequently contaminates rice products and produces cereulide toxins, presenting a significant risk to food safety and human health. In contrast, Bacillus subtilis is a promising source of antimicrobial peptides (AMPs). In this research, a novel AMP named BCP4 (KGKTLLQ) was discovered through the fermentation of shrimp waste with B. subtilis, which speculated that BCP4 might be generated through enzymatic hydrolysis catalyzed by endogenous enzymes naturally present in shrimp waste. BCP4 demonstrated potent antibacterial activity against B. cereus with a minimum bactericidal concentration (MBC) of 62.5 μg/mL and bacterial time-kill of 3 h. BCP4 surpassed the bactericidal efficiency of nisin (500 μg/mL), a commonly used AMP of microbial origin. BCP4 operates by causing damage to the bacterial cell wall and membrane, which allows the contents of the cell to flow out. BCP4 also penetrates the cell membrane and binds with DNA, effectively sterilizing the bacteria. Meanwhile, treatment of BCP4 with mammalian red blood cells revealed that it was nonhemolytic. Furthermore, the growth of B. cereus in rice porridge was significantly inhibited by BCP4 at a concentration of 62.5 μg/mL. This study provides a theoretical basis for using BCP4 to control B. cereus contamination.