Gayathree Murugappan, Mark Sikder, Sergio Vaccari, Debra Minjarez, Nam Tran, Julia Kim, Peter Klatsky
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引用次数: 0
Abstract
Objective: To compare embryologic and clinical outcomes following oocyte cryopreservation (OC) and immediate fertilization for embryo cryopreservation (EC) in non-infertile patients.
Design: Retrospective cohort study SUBJECTS: Patients treated at a single fertility center from 2016 to 2023.
Exposure: OC MAIN OUTCOME MEASURES: Blastocyst development was compared between women undergoing OC and non-infertile women undergoing immediate fertilization for EC between 18 and 42 years of age. ICSI, PGT-A, and eSET were used in all cycles.
Results: Outcomes from 3697 thawed MII oocytes obtained from 239 OC patients were compared to 28,959 fresh MII oocytes obtained from 2031 patients undergoing immediate fertilization for EC. Average thaw MII survival rate was 92% and high across age groups. Post-thaw oocytes demonstrate lower fertilization rate compared to fresh oocytes (85% v 90%, p < 0.05). As oocyte age increased, the mean number of MII used per blastocyst increased to a greater extent among thawed compared to fresh oocytes (2.9 v 2.2 MII age 18-34, 3.3 v 2.5 MII age 34-37, 3.9 v 2.9 MII age 38-40, 7.5 v 3.9 MII age 41-42, p < 0.05). Among patients achieving transfer of a euploid blastocyst, clinical pregnancy rate and live birth rate per transfer were similar using thawed and fresh oocytes (p = 0.82 and p = 0.79, respectively).
Conclusions: Blastocyst conversion is lower from thawed oocytes compared to fresh oocytes. Differences in blastocyst development between thawed and frozen oocytes increase with advancing oocyte age, suggesting that older oocytes may be less efficient in their conversion to blastocysts after vitrification and warming.
目的：比较非不孕症患者卵母细胞冷冻保存（OC）和胚胎冷冻保存（EC）后立即受精的胚胎学和临床结果。设计：回顾性队列研究对象：2016年至2023年在单一生育中心治疗的患者。主要观察指标：比较18 - 42岁接受体外受精的女性和接受体外受精的非不育女性的囊胚发育情况。所有周期均采用ICSI、PGT-A和eSET。结果：来自239名OC患者的3697个解冻的MII卵母细胞与来自2031名接受EC立即受精的患者的28959个新鲜的MII卵母细胞的结果进行了比较。解冻期MII的平均存活率为92%，各年龄组均较高。与新鲜卵母细胞相比，解冻后的卵母细胞受精率较低（85% vs 90%）。结论：解冻后的卵母细胞与新鲜卵母细胞相比，囊胚转化较低。随着卵母细胞年龄的增长，解冻卵母细胞和冷冻卵母细胞之间囊胚发育的差异增加，这表明年龄较大的卵母细胞在玻璃化和加热后向囊胚转化的效率可能较低。
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
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