Electrochemical determination of ascorbic acid using sensitive and disposable methylene blue modified pencil graphite electrode.

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-12-03 DOI:10.1016/j.ab.2024.115733

K G Aishwarya, Y Arthoba Nayaka, E Pradeepa, H R Sahana

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引用次数: 0

Abstract

In the present work, a convenient, efficient and disposable electrochemical sensor has been developed by electropolymerizing methylene blue (PMB) on the surface of a pencil graphite electrode (PGE), which facilitates the electrochemical analysis of an antioxidant l-Ascorbic Acid (AA). The structural characteristics of both the methylene blue modified pencil graphite electrode (PMB/PGE) and the bare pencil graphite electrode (BPGE) have been examined using scanning electron microscopy (SEM) in conjunction with energy-dispersive X-ray analysis (EDX). Additionally, the charge transfer behavior has been evaluated using the electron impedance spectroscopy (EIS). The voltammetric response of AA has been examined using different methods, such as differential pulse voltammetry (DPV) and linear sweep voltammetry (LSV). This exploration has been carried out in 0.1 M phosphate buffer solution (PBS) of physiological pH 7.0. The electrochemical sensor PMB/PGE proposed in this study exhibited an improved peak current and a slight negative shift in peak potential for AA compared to bare electrode. The enhancement in peak current at the modified electrode has been attributed to the electrocatalytic characteristics of the modifiers. The limit of detection (LOD) for AA has been determined using the differential pulse voltammetry (DPV), with concentrations ranging from 1.0 μM to 12.0 μM. The calculated LOD value has been found to be 0.15 μM. The selectivity and practicality of the modified electrode has been assessed through the real sample analysis and demonstrating its capability to detect AA in the presence of paracetamol (PA) resulting in satisfactory recovery results. Hence the proposed sensor could be successfully validated for the determination of AA in pharmaceutical sample.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.