Boron-Doped Nano Hydroxyapatite Grafts for Bone Regeneration in Rat Mandibular Defects.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Berat Baturay Demirkiran, Zeynep Deniz Sahin Inan, Rasim Hamutoğlu, Kerim Emre Öksüz, Zekiye Hasbek, Emine Elif Altuntaş
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引用次数: 0

Abstract

The aim of this study was to evaluate the potential effects of boron-doped nano hydroxyapatite grafts on craniofacial bone regeneration in critical bone defects in the mandibular corpus of rats, in terms of scintigraphic and histopathological aspects. Forty Wistar albino rats, with an average weight of 200-220 g, aged 16-18 weeks, and all male, were used in the study. The rats were randomly assigned to five groups, each containing 8 rats, as follows: group C1 (no procedure applied to the mandible), group C2 (surgical defect created in the mandible but no treatment applied), group nHA (nano hydroxyapatite applied to the surgical defect area), group nHA + B1 (nano hydroxyapatite + 1% boron applied to the surgical defect area), and group nHA + B2 (nano hydroxyapatite + 2% boron applied to the surgical defect area). A standard 4 × 4 mm full-thickness transosseous bone defect was created in the mandibular corpus of all rats, except for those in group C1. The bone defect in the rats in group C2 was left to heal naturally. Nano hydroxyapatite (nHA), nano hydroxyapatite + 1% boron, and nano hydroxyapatite + 2% boron were applied to the surgical defect areas of the other three groups, respectively. Bone scintigraphy was performed on all rats on days 0 (following the surgical procedure) and 28 of the experimental period. At the end of the 28th day, the animals were sacrificed, and tissue samples were collected for histological examination. A standard grading system was used to evaluate fracture healing. When the groups were compared in terms of bone healing histopathological scores, a statistically significant difference was observed between group C1 and the other groups (p < 0.005). In the statistical evaluation made according to the histopathological mean scores, the least improvement was observed in group C2. No statistically significant difference was observed between group nHA and group nHA + B1 and group C2 and between group nHA and group nHA + B1 in terms of bone healing scores (p > 0.005). A statistically significant difference was found between group nHA + B2 and group C2 (p = 0.026). Although there was no statistically significant difference in histopathological scores, the mean score closest to group C1 was observed in group nHA + B2. A statistically significant difference was observed between the groups in the scintigraphic evaluation performed on the 28th day of the experimental procedure, and the difference was between group C1 and group nHA + B1 and between group nHA and group nHA + B1 (p = 0.004; p = 0.028, p < 0.005). In the comparison of the values obtained on days 0 and 28 within the group, a statistically significant change was observed in group nHA + B1 and group nHA + B2 (p < 0.005). When the results of the present study were evaluated, it was thought that the boron-doped nHA graft biomaterials may have positive effects on bone healing. Providing a different perspective for the development of an alternative new treatment modality that can be locally applied in the treatment of fractures a serious and common health problem can be interpreted as an important outcome of the present study. We believe that this study will serve as a preliminary study for more comprehensive future studies on this subject.

掺硼纳米羟基磷灰石用于大鼠下颌骨缺损骨再生。
本研究旨在评价硼掺杂纳米羟基磷灰石移植物对颌骨严重骨缺损大鼠颅面骨再生的潜在影响,从影像学和组织病理学方面进行研究。本研究选用平均体重200-220克、年龄16-18周龄的Wistar白化大鼠40只,均为雄性。将大鼠随机分为5组,每组8只,分别为:C1组(下颌骨无手术处理)、C2组(下颌骨手术缺损未处理)、nHA组(纳米羟基磷灰石应用于手术缺损区)、nHA + B1组(纳米羟基磷灰石+ 1%硼应用于手术缺损区)、nHA + B2组(纳米羟基磷灰石+ 2%硼应用于手术缺损区)。除C1组外,其余大鼠下颌骨均形成标准的4 × 4 mm全层骨缺损。C2组骨缺损任其自然愈合。纳米羟基磷灰石(nHA)、纳米羟基磷灰石+ 1%硼和纳米羟基磷灰石+ 2%硼分别应用于其他三组手术缺损区域。在实验第0天(手术后)和第28天对所有大鼠进行骨显像。第28 d末处死大鼠,采集组织标本进行组织学检查。采用标准分级系统评价骨折愈合情况。两组骨愈合组织病理学评分比较,C1组与其他组比较差异有统计学意义(p 0.005)。nHA + B2组与C2组比较差异有统计学意义(p = 0.026)。虽然两组间组织病理学评分差异无统计学意义,但nHA + B2组的平均评分最接近C1组。在实验程序第28天进行的星图评估中,组间差异有统计学意义,其中C1组与nHA + B1组、nHA组与nHA + B1组间差异有统计学意义(p = 0.004;P = 0.028
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biological Trace Element Research
Biological Trace Element Research 生物-内分泌学与代谢
CiteScore
8.70
自引率
10.30%
发文量
459
审稿时长
2 months
期刊介绍: Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.
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