Study on Internal Standards Applicable to the Detection of Recombinant Erythropoietin.

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2024-12-05 DOI:10.1002/dta.3836

Chengshuai Niu, Kaifeng Liu, Xinchao Liu, Sen He, Xinmiao Zhou

引用次数: 0

Abstract

With the discovery of the variant c.577del in the EPO gene, the procedure for detecting the presence of recombinant erythropoietin (rEPO) has become complicated and time-consuming. To address this situation, an rEPO confirmation method that uses reverse-normal immunopurification coupled with western blotting (WB) and sodium N-lauroylsarcosinate polyacrylamide gel electrophoresis (SAR-PAGE), which can detect the EPO variant (VAR-EPO) and rEPO with anti-VAR-EPO and anti-EPO antibodies, has been developed. Therefore, it is necessary to develop an internal standard (IS) that can be recognized by an anti-VAR-EPO antibody to monitor reverse immunopurification, ensuring the reliability and accuracy of the analysis. In this study, we constructed an IS based on VAR-EPO modified with polyethylene glycol (PEG) and then assessed its reliability and applicability in doping analysis. The data provided here show that the obtained PEGylated VAR-EPO can be used as an IS to monitor the detection of not only rEPO and VAR-EPO but also EPO receptor agonists in urine samples.

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.