A facile optical fiber-embedded microfluidic biochip for rapid and sensitive detection of microRNA-let-7a in serum

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-12-06 DOI:10.1007/s00604-024-06865-5

Yongkai Lu, Jiaxin Xu, Siyan Liu, Baochun Tian, Feng Long

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Abstract

A novel hybridization chain reaction (HCR) powered optical fiber-embedded microfluidic biochip (HCR-FMB) has been constructed for ultrafast and sensitive detection of lethal-7a (let-7a) in serum. By integrating HCR, fluorescence energy resonant transfer, and evanescent wave fluorescence principle, the HCR-FMB enables detecting let-7a with satisfactory limit of detection of 100.0 pM within 6 min at room temperature, and demonstrates excellent specificity. The HCR-FMB can directly detect let-7a in serum with high sensitivity and without any pre-treatment, and good recoveries were observed for let-7a in serum samples, demonstrating their potential application to the analysis of serum samples. The HCR-FMB exhibits several advantages, including rapidity, enzyme-free, miniaturization, ease-of-operation, field-deployment applicability, minimal-equipment, and cost-effectiveness. The HCR-FMB can be considered a revolutionary detection device that rapidly adapts and deploys in various settings, especially in low medical resource regions.

Graphical Abstract

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一种快速灵敏检测血清中微rna -let-7a的光纤微流控生物芯片

构建了一种新型杂交链反应（HCR）驱动的光纤嵌入微流控生物芯片（HCR- fmb），用于超快速、灵敏地检测血清中致死-7a （let-7a）。HCR- fmb结合HCR、荧光能量共振转移和倏逝波荧光原理，在室温下6 min内检测let-7a，检出限为1000.0 pM，具有良好的特异性。HCR-FMB可直接检测血清中的let-7a，灵敏度高，无需任何预处理，血清样品中let-7a的回收率高，显示了其在血清样品分析中的潜在应用前景。HCR-FMB具有几个优点，包括快速、无酶、小型化、易于操作、现场部署适用性、最小的设备和成本效益。HCR-FMB可被视为一种革命性的检测设备，可在各种环境中迅速适应和部署，特别是在医疗资源匮乏的地区。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.