Characterization of deoxynivalenol dehydrogenase from Pelagibacterium sp. SCN 63–126 and its application

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-12-05 DOI:10.1007/s00203-024-04208-9

Wei Xu, Jiayi Yao, Jingbo Ma, Chen Lu, Chenfei Wang, Yurong Sun, Cuie Guang, Wanmeng Mu

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引用次数: 0

Abstract

Deoxynivalenol (DON), a type-B trichothecene mycotoxin, is primarily produced by Fusarium species and widely pollutes wheat and other grains. Enzymatic treatment of DON has been widely studied in recent years. Here, we present the biochemical identification of the DON dehydrogenase from Pelagibacterium sp. SCN 63–126 (Pe DDH). After removing the signal peptide, Pe DDH is effectively expressed in its soluble form. Biochemical identification indicates that the optimal temperature and pH of Pe DDH against DON is 35 ℃ and pH 8.5. Furthermore, Pe DDH is activated significantly in the presence of Ca²⁺, Mg²⁺, and Cu²⁺, and alternatively activated by pyrroloquinoline quinone (PQQ), phenazine methosulfate (PMS), and 2, 6-dichlorophenolindophenol (DCPIP). When PQQ, PMS, and DCPIP are combined, Pe DDH (60 µg/mL) effectively degrads DON (150 µM) in just 5 min, suggesting a synergistic effect of three cofactors on DON degradation. All these results suggest a great potential of Pe DDH in the control of DON contamination.

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Pelagibacterium sp. SCN 63-126脱氧雪腐镰刀菌醇脱氢酶的鉴定及其应用

脱氧雪腐镰刀菌醇（DON）是一种b型毛霉毒素，主要由镰刀菌产生，广泛污染小麦和其他谷物。酶法处理DON的研究近年来得到了广泛的研究。在此，我们报道了来自Pelagibacterium sp. SCN 63-126 （Pe DDH）的DON脱氢酶的生化鉴定。在去除信号肽后，Pe DDH以其可溶性形式有效表达。生化鉴定表明，Pe DDH对DON的最适温度和pH为35℃，pH为8.5。此外，Pe DDH在Ca2+， Mg2+和Cu2+存在下被显著激活，并被吡咯喹啉醌（PQQ），非那嗪甲硫代硫酸盐（PMS）和2,6 -二氯酚吲哚酚（DCPIP）交替激活。当PQQ、PMS和DCPIP联合使用时，Pe DDH（60µg/mL）在5 min内有效降解DON(150µM)，这表明三个辅因子对DON的降解具有协同作用。这些结果表明，Pe DDH在控制DON污染方面具有很大的潜力。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.