Tbx21 gene and its association with resistance against viral nervous necrosis (VNN) in Asian seabass, Lates calcarifer.

IF 5.8 2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology Pub Date : 2024-05-31 eCollection Date: 2024-11-01 DOI:10.1007/s42995-024-00234-0

Joey Wong, Zituo Yang, Le Wang, Fei Sun, Gen Hua Yue

{"title":"Tbx21 gene and its association with resistance against viral nervous necrosis (VNN) in Asian seabass, Lates calcarifer.","authors":"Joey Wong, Zituo Yang, Le Wang, Fei Sun, Gen Hua Yue","doi":"10.1007/s42995-024-00234-0","DOIUrl":null,"url":null,"abstract":"Viral nervous necrosis (VNN) caused by a betanodavirus (NNV) is one of the major diseases in Asian seabass (Lates calcarifer) hatcheries. Our previous studies showed that the tbx21 gene was in a QTL for NNV resistance in linkage group 23 in Asian seabass. The expression of this gene was changed in tissues of Asian seabass challenged with NNV. However, the role of tbx21 in NNV resistance remains largely unknown. In this study, tbx21 of Asian seabass was characterized. This gene consists of an ORF of 1866 bp, a 5' UTR of 357 bp, and a 3' UTR of 4674 bp. The TBX21 protein showed substantial amino acid similarity (70-96%) with other fish but exhibited lower identity (47-52%) with mammals. One SNP identified in the first intron was significantly associated with NNV resistance. In healthy fish, tbx21 was expressed in all tissues examined, and was highly expressed in the kidney and liver. The expression of tbx21 increased in the eye, gills, heart, kidney and gut, but decreased in the brain and spleen at five days after NNV challenge. Overexpression of tbx21 reduced the replication of NNV, whereas knockdown increased viral expression and virus titers. These results suggest that tbx21 plays a key role in NNV resistance. The SNP in this gene could be used as a marker to facilitate marker-assisted selection for NNV resistance. Further investigation of polymorphisms in the 5' and 3' UTRs of tbx21 may provide additional insights into the gene's role in NNV resistance.Supplementary information: The online version contains supplementary material available at 10.1007/s42995-024-00234-0.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 4","pages":"679-689"},"PeriodicalIF":5.8000,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602908/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Life Science & Technology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42995-024-00234-0","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Viral nervous necrosis (VNN) caused by a betanodavirus (NNV) is one of the major diseases in Asian seabass (Lates calcarifer) hatcheries. Our previous studies showed that the tbx21 gene was in a QTL for NNV resistance in linkage group 23 in Asian seabass. The expression of this gene was changed in tissues of Asian seabass challenged with NNV. However, the role of tbx21 in NNV resistance remains largely unknown. In this study, tbx21 of Asian seabass was characterized. This gene consists of an ORF of 1866 bp, a 5' UTR of 357 bp, and a 3' UTR of 4674 bp. The TBX21 protein showed substantial amino acid similarity (70-96%) with other fish but exhibited lower identity (47-52%) with mammals. One SNP identified in the first intron was significantly associated with NNV resistance. In healthy fish, tbx21 was expressed in all tissues examined, and was highly expressed in the kidney and liver. The expression of tbx21 increased in the eye, gills, heart, kidney and gut, but decreased in the brain and spleen at five days after NNV challenge. Overexpression of tbx21 reduced the replication of NNV, whereas knockdown increased viral expression and virus titers. These results suggest that tbx21 plays a key role in NNV resistance. The SNP in this gene could be used as a marker to facilitate marker-assisted selection for NNV resistance. Further investigation of polymorphisms in the 5' and 3' UTRs of tbx21 may provide additional insights into the gene's role in NNV resistance.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-024-00234-0.

查看原文本刊更多论文

亚洲海鲈Tbx21基因及其与病毒性神经坏死（VNN）抗性的关系

由betanodavirus （NNV）引起的病毒性神经坏死（VNN）是亚洲海鲈（latates calcarifer）孵化场的主要疾病之一。我们的前期研究表明，tbx21基因位于亚洲海鱼第23连锁群的NNV抗性QTL中。NNV侵染后，该基因的表达发生了变化。然而，tbx21在NNV耐药中的作用在很大程度上仍然未知。本研究对亚洲海鲈的tbx21进行了鉴定。该基因的ORF长度为1866 bp， 5′UTR长度为357 bp， 3′UTR长度为4674 bp。TBX21蛋白与其他鱼类氨基酸相似性较高（70-96%），但与哺乳动物氨基酸相似性较低（47-52%）。在第一个内含子中发现的一个SNP与NNV抗性显著相关。在健康鱼类中，tbx21在所有检查组织中表达，并在肾脏和肝脏中高表达。在NNV刺激后第5天，tbx21在眼、鳃、心脏、肾脏和肠道中的表达增加，但在脑和脾脏中的表达减少。tbx21的过表达降低了NNV的复制，而敲低则增加了病毒的表达和病毒滴度。这些结果表明tbx21在NNV抗性中起关键作用。该基因的SNP可作为标记，促进NNV抗性的标记辅助选择。对tbx21的5‘和3’ utr多态性的进一步研究可能为该基因在NNV抗性中的作用提供更多的见解。补充信息：在线版本包含补充资料，可在10.1007/s42995-024-00234-0获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Marine Life Science & Technology MARINE & FRESHWATER BIOLOGY-

CiteScore

9.60

自引率

10.50%

发文量

期刊介绍： Marine Life Science & Technology (MLST), established in 2019, is dedicated to publishing original research papers that unveil new discoveries and theories spanning a wide spectrum of life sciences and technologies. This includes fundamental biology, fisheries science and technology, medicinal bioresources, food science, biotechnology, ecology, and environmental biology, with a particular focus on marine habitats. The journal is committed to nurturing synergistic interactions among these diverse disciplines, striving to advance multidisciplinary approaches within the scientific field. It caters to a readership comprising biological scientists, aquaculture researchers, marine technologists, biological oceanographers, and ecologists.