A Feasible Method for Vein Puncture and Drug Administration in Rats: Ultrasound-guided Internal Jugular Vein Puncture

IF 2.4 3区医学 Q2 ACOUSTICS

Ultrasound in Medicine and Biology Pub Date : 2024-11-30 DOI:10.1016/j.ultrasmedbio.2024.11.013

Cai Hu , Huahui Liu , Zhengrong Lin , Shuang Liang , Qiqi Liu , Erjiao Xu

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引用次数: 0

Abstract

Objective

In the majority of animal experiments, vein puncture is necessary for the drugs administration. This study aimed to propose a new vein puncture method, ultrasound-guided internal jugular vein (IJV) puncture, and compare it with the traditional tail vein puncture.

Methods

We divided 24 male SpragueDawley rats randomly into 2 groups: 12 rats in the tail vein puncture group and other 12 rats in the ultrasound-guided IJV puncture group. After successful puncture, rats from two groups were injected with 0.1 mL ultrasound contrast agents. The average puncture time, the success rate of the first puncture, and the imaging effects of contrast-enhanced ultrasound in the sciatic nerve and liver parenchyma of rats after injecting ultrasound contrast agents were evaluated using time–intensity curves for both different puncture methods.

Results

The average puncture time of the ultrasound-guided IJV group was lower than that of the tail vein puncture group (p = 0.013), and the success rate of the first puncture was significantly higher than that of the tail vein puncture group (p = 0.037). There were no significant differences in the imaging effects of contrast-enhanced ultrasound on the sciatic nerve and liver parenchyma between the two different puncture methods. Additionally, neither of the two puncture methods resulted in obvious symptoms such as hematoma formation, convulsions, restlessness or even death in rats.

Conclusions

Ultrasound-guided IJV puncture could be a safe, effective method with a high success rate for rat vein puncture and drug administration, which could be an alternative to rat tail vein puncture.

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一种可行的大鼠静脉穿刺给药方法：超声引导颈内静脉穿刺。

目的：在大多数动物实验中，静脉穿刺是给药的必要手段。本研究旨在提出一种新的静脉穿刺方法——超声引导颈内静脉穿刺（IJV），并与传统的尾静脉穿刺进行比较。方法：将24只雄性SpragueDawley大鼠随机分为2组：尾静脉穿刺组12只，超声引导下IJV穿刺组12只。穿刺成功后，两组大鼠均注射超声造影剂0.1 mL。采用时间-强度曲线评价超声造影剂注射后大鼠坐骨神经及肝实质的平均穿刺时间、首次穿刺成功率及超声增强成像效果。结果：超声引导下IJV组平均穿刺时间低于尾静脉穿刺组（p = 0.013），首次穿刺成功率显著高于尾静脉穿刺组（p = 0.037）。对比增强超声对坐骨神经及肝实质的成像效果在两种穿刺方式间无显著差异。此外，两种穿刺方法均未引起大鼠血肿形成、惊厥、躁动甚至死亡等明显症状。结论：超声引导下IJV穿刺是一种安全、有效、成功率高的大鼠静脉穿刺和给药方法，可作为大鼠尾静脉穿刺的替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ultrasound in Medicine and Biology 医学-核医学

CiteScore

6.20

自引率

6.90%

发文量

325

审稿时长

70 days

期刊介绍： Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.