Evaluation of virulence genes in Proteus strains isolated from diabetic foot infections and urinary tract infections.

IF 1.4 4区医学 Q4 INFECTIOUS DISEASES

Journal of Infection in Developing Countries Pub Date : 2024-10-31 DOI:10.3855/jidc.18928

Ruth M Phiri, Mustafa Bülent Ertuğrul, Bülent Bozdoğan, Tuğrul Hoşbul

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引用次数: 0

Abstract

Introduction: Proteus species are frequently isolated from urinary tract infections (UTIs) and diabetic foot infections (DFIs). We aimed to evaluate the presence of virulence genes in P. mirabilis and P. vulgaris strains isolated from DFI and UTI.

Methodology: A total of 78 Proteus isolates (57 P. mirabilis and 21 P. vulgaris) collected from patients were studied. The isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The presence of virulence-associated genes (hlyA, mrpA, atfA, pmfA, hmpA, ptaA, ureA, ureC, zapA, ireA, rsbA, flaA, and ucaA) was evaluated by polymerase chain reaction (PCR).

Results: 46 isolates were obtained from wound cultures, and 32 were obtained from the midstream urine cultures. All virulence genes, except hlyA, were detected in the study. ureA was the most detected gene in both UTI (100%) and DFI isolates (84.8%). The distributions of ureC, flaA, hpmA, ireA, rsbA, pmfA, zapA, ucaA, ptaA, atfA, and mrpA genes in DFI and UTI isolates were as follows: 82.6% and 96.9%, 71.7% and 93.8%, 69.6% and 93.8%, 69.6% and 96.9%, 69.6% and 96.9, 67.4% and 81.3%, 65.2% and 43.8%, 54.3 and 71.9%, 34.8% and 96.9%, 26.1 and 93.8%, and 23.9% and 46.9%, respectively.

Conclusions: We demonstrated that P. mirabilis and P. vulgaris express a variety of virulence genes related to pathogenicity. All virulence genes were found to be more frequent in UTI isolates except zapA. There is limited data on the virulence factors of Proteus species in DFIs. Further studies are needed to investigate virulence genes in wound isolates.

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糖尿病足和尿路感染分离的变形杆菌毒力基因评价。

变形杆菌经常从尿路感染（uti）和糖尿病足感染（dfi）中分离出来。我们的目的是评估从DFI和UTI分离的P. mirabilis和P. vulgaris菌株的毒力基因的存在。方法：对采集的78株变形杆菌进行分析，其中奇异变形杆菌57株，寻常变形杆菌21株。采用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱仪对分离物进行鉴定。采用聚合酶链反应（PCR）法检测毒力相关基因（hlyA、mrpA、atfA、pmfA、hmpA、ptaA、ureA、ureC、zapA、ireA、rsbA、flaA、ucaA）的存在情况。结果：伤口培养分离46株，中游尿培养分离32株。除hlyA外，其余毒力基因均检测到。尿素是UTI和DFI分离株中检出最多的基因，分别为100%和84.8%。ureC、flaA、hpmA、ireA、rsbA、pmfA、zapA、ucaA、ptaA、atfA和mrpA基因在DFI和UTI分离株中的分布分别为：82.6%和96.9%、71.7%和93.8%、69.6%和93.8%、69.6%和96.9%、69.6%和96.9、67.4%和81.3%、65.2%和43.8%、54.3和71.9%、34.8%和96.9%、26.1和93.8%、23.9%和46.9%。结论：我们证明了P. mirabilis和P. vulgaris表达了多种与致病性相关的毒力基因。除zapA外，所有毒力基因在UTI分离株中更为常见。关于变形杆菌种类在dfi中的毒力因子的数据有限。需要进一步研究伤口分离株的毒力基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection in Developing Countries INFECTIOUS DISEASES-

CiteScore

3.70

自引率

5.30%

发文量

239

审稿时长

4-8 weeks

期刊介绍： The Journal of Infection in Developing Countries (JIDC) is an international journal, intended for the publication of scientific articles from Developing Countries by scientists from Developing Countries. JIDC is an independent, on-line publication with an international editorial board. JIDC is open access with no cost to view or download articles and reasonable cost for publication of research artcles, making JIDC easily availiable to scientists from resource restricted regions.