Control and Disposal of Invasive Japanese Knotweed Reynoutria japonica Houtt. Using Microwave Treatment.

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Krzysztof Słowiński, Beata Grygierzec, Sylwester Tabor, Szymon Bucior, Agnieszka Synowiec
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Abstract

The study aims to assess the effectiveness of microwave treatment (MWT) at a frequency of 2.45 GHz and a power of 800 W to control Japanese knotweed (Reynoutria japonica Houtt.) using a self-propelled device that was built in the in-house facility. The MWT was applied in the field population of knotweed in July 2022. First, plants were mechanically moved from the area of 1 m2, and next, the cut shoots around 4 cm high were microwave-treated for 25 min, 20 min, and 15 min. The control treatments were: 1) only cut plants and 2) rhizomes dug out to 30 cm deep. The effectiveness of the microwave treatments was observed for the next 11 months by counting the number of newly grown shoots. The results showed that a 25 min MWT was 100% effective in Japanese knotweed loss of vitality, while a 15 min MWT microwave treatment stimulated plant growth by around 50%, compared to controls. Rhizomes were dug out in a separate in vitro experiment for laboratory testing. The rhizomes were categorized by thickness and subjected to a 60 s MWT using a commercial microwave, after which their temperature and vitality were assessed. The temperature of rhizomes following MWT depended on their thickness. Those rhizomes that warmed to temperatures above 42 °C were effectively destroyed. Summing up, the time plants are exposed to microwaves plays a major role in the effectiveness of this method. The longer the exposure to MWT, the better control. The thinner the rhizomes, the more effective the in vitro MWT rhizomes disposal.

入侵日本虎杖的防治。使用微波处理。
该研究旨在评估频率为2.45 GHz、功率为800 W的微波处理(MWT)对日本结须(Reynoutria japonica Houtt.)的控制效果,使用的是在内部设施中建造的自推进装置。2022年7月,将MWT应用于结缕草田间种群。首先将植株机械移出1 m2,然后对4 cm左右高的插枝进行微波处理25 min、20 min和15 min。对照处理为:1)只插枝,2)根状茎挖至30 cm深。在接下来的11个月里,通过计算新芽的数量来观察微波处理的有效性。结果表明,与对照相比,25 min微波处理对日本虎杖活力丧失的效果为100%,而15 min微波处理对植物生长的刺激约为50%。在一个单独的体外实验中挖出根茎进行实验室测试。根据根状茎的厚度对其进行分类,并利用商用微波对其进行60 s的MWT处理,然后对其温度和活力进行评估。MWT后根茎温度与根茎厚度有关。那些加热到42°C以上的根茎被有效地破坏了。综上所述,植物暴露在微波中的时间对该方法的有效性起着重要作用。接触MWT时间越长,控制效果越好。根茎越细,离体MWT根茎处理效果越好。
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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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