CatWalk XT Gait Parameters Associated with Mouse Achilles Tendon Injury and Healing.

IF 0.5 Q4 ORTHOPEDICS
Giulia Crosio, Emily R King, Alice H Huang
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引用次数: 0

Abstract

Tendons are connective tissues with limited healing potential which results in permanently impaired function. Although direct mechanical testing of tendon remains the gold standard for functional analyses, this assay is terminal and tracking healing over time requires the use of many animals. An alternative method for quantifying tendon function is gait analysis, which is non-terminal and enables longitudinal tracking of the same animal. To date, commercial systems used to analyze gait are mostly applied to study neurobehavior, and applications for tendon research has been limited. Since these systems typically output many parameters, it is challenging to know which parameter is most relevant for a specific injury model. To address this challenge, we used a well-established rodent locomotion system (CatWalk XT) to measure longitudinal gait parameters in sham and Achilles tendon-injured mice (from PREOP to 56 days post-injury) and identified relevant and reproducible parameters specifically associated with Achilles tendon injury and healing. Micro computed tomography (micro-CT) and mechanical testing also confirmed persistent tendon impairment in the same animals at the terminal timepoint. Collectively, our results provide a useful reference and recommendation of CatWalk gait parameters and the control comparisons that both conserve the use of animals while maintaining high reproducibility standards for Achilles tendon injury studies.

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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
51
期刊介绍: MLTJ (Muscle, Ligaments and Tendons Journal) is an open access, peer-reviewed online journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal, ligament, tendon, public health, exercise physiology and kinesiology issues. Muscle, Ligaments and Tendons Journal (MLTJ) provides the platform for exchange of new clinical and scientific information in the most precise and expeditious way to achieve timely dissemination of information and cross-fertilization of ideas. It is the official journal of the Italian Society of Muscles, Ligaments and Tendons (I.S.Mu.L.T.), Società Italiana Terapia con Onde D’urto (S.I.T.O.D.) and Società Italiana Studio Piede e Caviglia (S.I.S.P.E.C)
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