Effect of exogenous IBA on root morphology and endogenous hormone Metabolic pathways in castor seedlings under Pb/Zn stress

Abstract

Ricinus communis, a plant of significant industrial value for its oil, is renowned for its robust root system and vigorous growth, qualities that render it an exemplary candidate for the ecological remediation of soils contaminated with heavy metals. The impact of heavy metal stress on root development is characterized by inhibition, a phenomenon whose underlying mechanisms are not fully elucidated. To shed light on this, a study was conducted wherein varying concentrations of the exogenous auxin, IBA, were applied to the roots of Ricinus communis to scrutinize its influence on the endogenous indole-3-acetic acid (IAA) metabolism in seedlings and to delineate the molecular underpinnings of its effects on root morphology. It was observed that IBA significantly amplified the total root surface area by a factor of 1.29 and increased the number of root tips by 40.11% under lead (Pb) stress, and by 32.29% and 91.19%, respectively, under zinc (Zn) stress. These findings underscore the efficacy of IBA in promoting the proliferation of lateral roots in seedlings subjected to stress induced by either Pb or Zn. Further analysis of auxin signaling pathways revealed that the presence of Pb or Zn impedes root growth and lateral root formation by perturbing auxin transporters and signaling molecules. Notably, IBA was found to foster the development of lateral roots by modulating the expression of specific transporters. Post-application of IBA, the endogenous levels of IAA in roots exhibited a 2.80-fold elevation under Pb stress, IBA stimulated the activity of key biosynthetic enzymes, such as RcNIT and RcTAR, culminating in elevated IAA levels. Conversely, under Zn stress, IBA was observed to diminish the levels of RcTAR, which in turn led to reduced IAA levels. These outcomes contribute to a deeper comprehension of the modulatory role of IBA in the context of heavy metal stress.