Do different wheat ploidy levels respond differently against stripe rust infection: Interplay between reactive oxygen species (ROS) and the antioxidant defense system?

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-02-01 DOI:10.1016/j.plaphy.2024.109259

Farkhandah Jan , Parthiban M , Satinder Kaur , Mohd Anwar Khan , Farooq Ahmad Sheikh , Fehim Jeelani Wani , A.A. Saad , Yogita Singh , Upendra Kumar , Vikas Gupta , Mahendar Thudi , Dinesh K. Saini , Sundeep Kumar , Rajeev Kumar Varshney , Reyazul Rouf Mir

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引用次数: 0

Abstract

Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst) is the most damaging wheat disease, causing substantial losses in global wheat production and productivity. Our study aimed to unravel the complex reciprocity between reactive oxygen species and the antioxidant defense system as a source of resistance against stripe rust in diploid, tetraploid and hexaploid wheat genotypes. The significant genetic variability for stripe rust in the materials under study was evident as the genotypes showed contrasting responses during both the adult and seedling stages. Our thorough perspective on the biochemical responses of wheat genotypes to stripe rust infection revealed distinct patterns in oxidative damage, antioxidant enzymes and photosynthetic pigments. Principal component analysis revealed inverse correlations between antioxidants and ROS, underscoring their key function in maintaining the cellular redox balance and protecting plants against oxidative damage. Diploid (Ae. tauschii) wild wheat exhibited a better biochemical defense system and greater resistance to stripe rust than the tetraploid (T. durum) and hexaploid (Triticum aestivum) wheat genotypes. The antioxidant enzyme activity of durum wheat was moderate compared to diploid and hexaploid wheat genotypes. The hexaploid wheat genotypes exhibited increased ROS production, reduced antioxidant enzyme activity and decreased photosynthetic pigment levels. This study enhances understanding of the antioxidant defense system across different wheat ploidies facing stripe rust, serving as a valuable strategy for improving crop disease resistance. This study validated the biochemical response of stripe rust-resistant and susceptible candidate genotypes, which will be used to develop genetic resources for discovering stripe rust resistance genes in wheat.

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不同的小麦倍性水平对条锈病的反应是否不同：活性氧（ROS）与抗氧化防御系统之间的相互作用？

小麦条锈病（锈病）是小麦最具破坏性的病害，对全球小麦产量和生产力造成重大损失。本研究旨在揭示二倍体、四倍体和六倍体小麦抗条锈病基因型中活性氧与抗氧化防御系统之间复杂的相互作用。研究材料中条锈病的遗传变异显著，在成虫期和幼苗期表现出不同的基因型反应。我们对小麦基因型对条锈病的生化反应进行了深入的研究，揭示了小麦在氧化损伤、抗氧化酶和光合色素方面的不同模式。主成分分析揭示了抗氧化剂与活性氧之间的负相关关系，强调了它们在维持细胞氧化还原平衡和保护植物免受氧化损伤方面的关键功能。二倍体(Ae。与四倍体（T. durum）和六倍体（Triticum aestivum）基因型相比，tauschii野生小麦具有更好的生化防御系统和更强的抗条锈病能力。与二倍体和六倍体小麦基因型相比，硬粒小麦抗氧化酶活性中等。六倍体小麦基因型表现出ROS生成增加、抗氧化酶活性降低和光合色素水平降低的特点。本研究加深了对小麦不同倍体抗条锈病防御系统的认识，为提高作物抗病能力提供了有价值的策略。本研究验证了小麦条锈病抗性和易感候选基因型的生化反应，为发现小麦条锈病抗性基因开发遗传资源奠定基础。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.