The first mitogenome of the genus Amphalius (Siphonaptera: Ceratophyllidae) and its phylogenetic implications.

IF 2.1 3区 医学 Q2 PARASITOLOGY
Parasitology Pub Date : 2024-09-01 Epub Date: 2024-12-03 DOI:10.1017/S0031182024000635
Ju Pu, Xiaoxia Lin, Wenge Dong
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引用次数: 0

Abstract

Amphalius spirataenius belongs to Arthropoda, Insecta, Siphonaptera, Ceratophylloidea, Ceratophyllinae, Amphalius. Only 2 species from the subfamily Ceratophyllinae have been sequenced for mitogenomes to date. The genus Amphalius mitogenome research was still blank. The A. spirataenius mitogenome was determined, annotated and analysed for the first time in this study. The 14 825 bp long genome has the typical metazoan of 37 genes with insect ancestral genome arrangement pattern. There was no significant difference in codon usage of 13 protein-coding genes: UUA, UCU, GUU, ACU and GCU were the most frequently used codons. It was found that the reason for codon preference mainly contributed to natural selection base on PR2, ENC-plot and neutrality curve analysis. Evolutionary rate, conserved sites, variable sites and nucleotide diversity analysis indicated that nad6 of A. spirataenius had the fastest evolutionary rate, while cox1 had the slowest evolutionary rate. Phylogenetic trees were reconstructed based on 13 protein-coding genes and 2 rRNA genes datasets using Bayesian inference and maximum likelihood method. The phylogenetic tree supported that both Siphonaptera and Mecoptera were monophyletic, and were sister groups to each other. This study filled gap of the genus Amphalius mitogenome sequences and was of great significance for understanding evolution of the order Siphonaptera.

双翅虫属的第一个有丝分裂基因组及其系统发育意义。
螺旋藻属节肢动物、昆虫亚目、吸翅目、角藻亚目、角藻亚目、角藻亚目。迄今为止,仅有2种角鼻苔亚科植物进行了有丝分裂基因组测序。对双足属有丝分裂基因组的研究尚属空白。本研究首次对螺旋藻有丝分裂基因组进行了测定、注释和分析。全长14 825 bp的基因组具有37个基因的典型后生动物,具有昆虫祖先基因组排列模式。13个蛋白编码基因的密码子使用差异无统计学意义,UUA、UCU、GUU、ACU和GCU是使用频率最高的密码子。基于PR2、c -plot和中性曲线分析,发现密码子偏好的原因主要是自然选择。进化速率、保守位点、可变位点和核苷酸多样性分析表明,螺旋藻nad6进化速度最快,cox1进化速度最慢。基于13个蛋白质编码基因和2个rRNA基因数据集,利用贝叶斯推理和极大似然方法重构了系统发育树。系统发育树支持管翅目和甲翅目为单系,互为姊妹类群。该研究填补了双翅虫属有丝分裂基因组序列的空白,对了解管翅目昆虫的进化具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Parasitology
Parasitology 医学-寄生虫学
CiteScore
4.80
自引率
4.20%
发文量
280
审稿时长
3-8 weeks
期刊介绍: Parasitology is an important specialist journal covering the latest advances in the subject. It publishes original research and review papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in parasite biochemistry, molecular biology and genetics, ecology and epidemiology in the context of the biological, medical and veterinary sciences. Included in the subscription price are two special issues which contain reviews of current hot topics, one of which is the proceedings of the annual Symposia of the British Society for Parasitology, while the second, covering areas of significant topical interest, is commissioned by the editors and the editorial board.
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