Interstitial Cells of Cajal Are Required for Different Intestinal Motility Responses Induced by Acupuncture.

IF 3.5 3区医学 Q1 CLINICAL NEUROLOGY

Neurogastroenterology and Motility Pub Date : 2024-12-01 DOI:10.1111/nmo.14973

Longhua Du, Qingguang Qin, Xun He, Xiaoxi Wang, Guang Sun, Bing Zhu, Kun Liu, Xinyan Gao

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引用次数: 0

Abstract

Background: The movement of intestinal smooth muscle is regulated by the external autonomic nervous system (ANS) and its internal enteric nervous system (ENS). Previous studies have shown that acupuncture has a bidirectional regulating effect on intestinal motility through the sympathetic and vagal ANSs. ENS can independently regulate the sensory, secretory, and motor functions of the intestine. The interstitial cells of Cajal (ICC), the pacemaker cells in ENS, play a key role in maintaining gastrointestinal motility. However, studies on the role and mechanism of ICC in the regulation of intestinal function by acupuncture are still unclear.

Methods: To investigate the effect of ICC on the regulation of intestinal motility by manual acupuncture (MA), we recorded the pressure of warm water-filled manometric balloons in duodenum, jejunum, and distal colon in ICC deficiency WsWs^-/- rats and wild-type littermates WsRC^+/+ rats, and performed MA at ST25 (Tianshu), ST37 (Shangjuxu), LI11 (Quchi), and BL25 (Danchangshu) acupoints. Furthermore, the excretion of phenol red in feces before and after MA at ST37 or ST25 was assessed.

Key result: In WsRC^+/+ rats, MA at ST37, LI11, and BL25 promoted duodenal, jejunal, and distal colon motility, whereas MA at ST25 significantly inhibited duodenal and jejunal motility and promoted distal colon motility. ICC deficiency in WsWs^-/- rats led to a reduction in the promoting effect of LI11 on duodenal motility, a decrease in the promoting effect of ST37 on jejunal motility, and a significant reduction in the promoting effect of BL25 on distal colonic motility in those rats. Additionally, ICC absence significantly attenuated the inhibitory effect of ST25 on duodenal motility. MA at ST37 or ST25 did not change the content of phenol red in the feces in WsRC^+/+ and WsWs^-/- rats.

Conclusion and inferences: Our results suggest that the absence of ICC impairs the bidirectional regulatory effect of MA on intestinal function. It reveals the important role of ICC in the treatment of intestinal dysfunction diseases by acupuncture and provides a new theoretical basis for the treatment of such diseases by MA.

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针刺诱导不同肠道运动反应需要Cajal间质细胞。

背景：肠道平滑肌的运动受外自主神经系统（ANS）及其内肠神经系统（ENS）的调控。已有研究表明，针刺通过交感神经和迷走神经对肠道运动具有双向调节作用。ENS可以独立调节肠道的感觉、分泌和运动功能。Cajal间质细胞（ICC）是ENS的起搏器细胞，在维持胃肠运动中起关键作用。然而，对ICC在针刺调节肠道功能中的作用和机制的研究尚不清楚。方法：为研究ICC对手针刺（MA）调节肠道运动的作用，我们记录ICC缺乏型wws -/-大鼠和野生型窝鼠WsRC+/+大鼠十二指肠、空肠和结肠远端充满温水的测压球的压力，并在ST25（天枢）、ST37（上巨虚）、LI11（曲池）和BL25（胆常枢）穴位进行MA。此外，还对ST37或ST25 MA前后粪便中酚红的排泄量进行了评估。关键结果：在WsRC+/+大鼠中，ST37、LI11和BL25的MA促进了十二指肠、空肠和结肠远端运动，而ST25的MA显著抑制了十二指肠和空肠运动，促进了结肠远端运动。wws -/-大鼠的ICC缺乏导致LI11对十二指肠运动的促进作用减弱，ST37对空肠运动的促进作用减弱，BL25对远端结肠运动的促进作用显著降低。此外，缺乏ICC显著减弱了ST25对十二指肠运动的抑制作用。ST37或ST25 MA未改变WsRC+/+和WsWs-/-大鼠粪便中酚红的含量。结论和推论：我们的研究结果表明，缺乏ICC会损害MA对肠道功能的双向调节作用。揭示了ICC在针刺治疗肠功能障碍疾病中的重要作用，为MA治疗此类疾病提供了新的理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neurogastroenterology and Motility 医学-临床神经学

CiteScore

7.80

自引率

8.60%

发文量

178

审稿时长

3-6 weeks

期刊介绍： Neurogastroenterology & Motility (NMO) is the official Journal of the European Society of Neurogastroenterology & Motility (ESNM) and the American Neurogastroenterology and Motility Society (ANMS). It is edited by James Galligan, Albert Bredenoord, and Stephen Vanner. The editorial and peer review process is independent of the societies affiliated to the journal and publisher: Neither the ANMS, the ESNM or the Publisher have editorial decision-making power. Whenever these are relevant to the content being considered or published, the editors, journal management committee and editorial board declare their interests and affiliations.