Electroacupuncture at Zusanli (ST36) Alleviate Intestinal Ischemia-Reperfusion Injury by Regulating the Cholinergic-miRNA 124 Pathway.

IF 3.5 3区医学 Q1 CLINICAL NEUROLOGY

Neurogastroenterology and Motility Pub Date : 2024-12-01 DOI:10.1111/nmo.14971

YanXia Geng, Hai Lv, Yu Liu, DingDing Guo, JingJing Zheng, YingXin Li, Hua Jiang, Dong Chen

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引用次数: 0

Abstract

Objective: Intestinal ischemia-reperfusion injury (IIRI) is common in a variety of critical diseases and acute stress, and acupuncture is a promising treatment for IIRI. The aim of this study is to explore the mechanism of electroacupuncture (EA) at Zusanli (ST36) in improving IIRI from the perspective of the cholinergic anti-inflammatory pathway (CAP) and miRNA 124.

Methods: Seven groups of mice were performed: Control group, I/R group (IIRI model), EA group (I/R + EA), miRNA mimic group (I/R + EA + miRNA 124 mimic), miRNA inhibitor group (I/R + EA + miRNA 124 inhibitor), PNU group (I/R + EA + α7nAChR agonist), and MLA group (I/R + EA+ α7nAChR antagonist). The expression of intestinal macrophages, α7nAChR, miRNA 124, and related molecules, including p-STAT3 and IL-6, as well as histological damage (Chiu's score) and mucosal permeability (serum FD4 concentration), were detected.

Results: The serum FD4 concentrations in the EA and PNU groups were significantly lower compared to the I/R group (p < 0.05). The expression of intestinal α7nAChR and miRNA 124 in the EA, miRNA mimic, and PNU groups was higher than that in the I/R group (p < 0.05), while levels of p-STAT3 and IL-6 were reduced (p < 0.05). Conversely, in the miRNA inhibitor and MLA groups, miRNA 124 levels were significantly lower than in the EA group (p < 0.05), and IL-6 levels were increased (p < 0.05).

Conclusion: EA at Zusanli may induce miRNA124 through the cholinergic pathway on intestinal macrophages, which may be a key neuro-immune target of EA in the treatment of IIRI.

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电针足三里（ST36）通过调节胆碱能- mirna 124通路减轻肠缺血再灌注损伤。

目的：肠缺血再灌注损伤（IIRI）常见于多种危重疾病和急性应激，针刺是治疗IIRI的一种很有前景的方法。本研究旨在从胆碱能抗炎通路（CAP）和miRNA 124的角度探讨电针（EA）足三里（ST36）改善IIRI的机制。方法：将小鼠分为7组：对照组、I/R组（IIRI模型）、EA组（I/R + EA）、miRNA模拟组（I/R + EA+ miRNA 124模拟）、miRNA抑制剂组（I/R + EA+ miRNA 124抑制剂）、PNU组（I/R + EA+ α7nAChR激动剂）和MLA组（I/R + EA+ α7nAChR拮抗剂）。检测大鼠肠道巨噬细胞α7nAChR、miRNA 124及相关分子p-STAT3、IL-6的表达，组织损伤（Chiu’s评分）、黏膜通透性（血清FD4浓度）。结果：EA组和PNU组血清FD4浓度明显低于I/R组(p)。结论：足三里EA可能通过胆碱能途径诱导肠巨噬细胞miRNA124，这可能是EA治疗IIRI的关键神经免疫靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neurogastroenterology and Motility 医学-临床神经学

CiteScore

7.80

自引率

8.60%

发文量

178

审稿时长

3-6 weeks

期刊介绍： Neurogastroenterology & Motility (NMO) is the official Journal of the European Society of Neurogastroenterology & Motility (ESNM) and the American Neurogastroenterology and Motility Society (ANMS). It is edited by James Galligan, Albert Bredenoord, and Stephen Vanner. The editorial and peer review process is independent of the societies affiliated to the journal and publisher: Neither the ANMS, the ESNM or the Publisher have editorial decision-making power. Whenever these are relevant to the content being considered or published, the editors, journal management committee and editorial board declare their interests and affiliations.