Establishment of a standardized daily behavior collection and analysis system for brain disease models of rhesus and cynomolgus monkeys and its application in autism spectrum disorder.
Xiaofeng Ren, Huimin Wang, Xiaoman Lv, Yi Zhou, Yingyin Fan, Yanjun Yu, Christoph W Turck, Yuhui Chen, Longbao Lv, Yingzhou Hu, Hao Li, Wenchao Wang, Dongdong Qin, Xiaoli Feng, Xintian Hu
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引用次数: 0
Abstract
Complex brain diseases seriously endanger human health, and early diagnostic biomarkers and effective treatments are currently lacking. Due to ethical constraints on human research, establishing monkey models is crucial to address these issues. With the rapid development of technology, transgenic monkey models of a range of brain diseases, especially autism spectrum disorder (ASD), have been successfully established. However, to establish practical and effective brain disease models and subsequently apply them to disease mechanism and treatment studies, there is still a lack of a standard tool, i.e., a system for collecting and analyzing the daily behaviors of brain disease model monkeys. Therefore, with the goal of undertaking a comprehensive and quantitative study of behavioral phenotypes, we established a standard daily behavior collection and analysis system, including behavioral data collection protocols and a monkey daily behavior ethogram (MDBE) for rhesus and cynomolgus monkeys, which are the most commonly used non-human primates in model construction. Then, we used ASD as an application example after referring to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (DSM-5-TR), which is widely used in clinical disease diagnosis to obtain ASD core clinical symptoms. We then established a sub-ethogram (ASD monkey core behavior ethogram (MCBE-ASD)) specifically for quantitative assessment of the core clinical symptoms of an ASD monkey model based on MDBE. Subsequently, we demonstrated the high reproducibility of the system.
期刊介绍:
Journal of Zheijang University SCIENCE B - Biomedicine & Biotechnology is an international journal that aims to present the latest development and achievements in scientific research in China and abroad to the world’s scientific community.
JZUS-B covers research in Biomedicine and Biotechnology and Biochemistry and topics related to life science subjects, such as Plant and Animal Sciences, Environment and Resource etc.