Spontaneous natural killer cell lymphoproliferative disorder in a rhesus macaque.

IF 1.2 3区 农林科学 Q3 VETERINARY SCIENCES
Marietta V Barro, Laura M Garzel, Rebekah I Keesler, Kerriann M Casey, Katherine J Olstad
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引用次数: 0

Abstract

Lymphoproliferative disorders of natural killer (NK)-cell lineage are well documented in humans but have yet to be documented in non-human primates (NHPs). Here we describe a case of NK-cell lymphoproliferative disorder/leukemia in a 20-y-old captive female rhesus macaque (Macaca mulatta). The animal clinically had mild splenomegaly and marked lymphocytosis with small-to-medium lymphocytes in blood smears. By flow cytometry and cluster differentiation, the lymphocytes were CD3-negative, CD8-positive, CD4-negative, and CD20-negative for cell surface markers; immunohistochemistry revealed the presence of intracellular CD3 and granzyme B. This immunoprofile is consistent with a NK-cell phenotype. Histologically, these cells were predominantly intravascular within the splenic red pulp, liver sinusoids, and to a lesser degree bone marrow. Oncogenic viruses, such as Mason-Pfizer monkey viruses (MPMV; formerly, and commonly known as, simian retroviruses or SRV; Retroviridae, Betaretrovirus maspfimon); simian immunodeficiency virus (SIV; Retroviridae, Lentivirus simimdef), and primate T-lymphotropic virus 1 (PTLV1; commonly known as simian T-lymphotropic virus type 1, STLV1; Retroviridae, Deltaretrovirus priTlym1), were not detected in this animal by serology. Immunohistochemistry using EBNA2 antibody to detect rhesus and cynomolgus monkey lymphocryptovirus (McGHV4/RLV and McGHV10 respectively; Orthoherpesviridae, Lymphocryptovirus macacinegamma4 and Lymphocryptovirus macacinegamma13, respectively) was negative. Together these findings are consistent with a diagnosis of naturally occurring NK-cell lymphoproliferative disorder. NK-cell lymphoproliferative disorder has not been reported previously in rhesus macaques, to our knowledge.

恒河猴自发性自然杀伤细胞淋巴增生性疾病。
自然杀伤(NK)细胞谱系的淋巴增生性疾病在人类中有很好的记录,但尚未在非人灵长类动物(NHPs)中有记录。在这里,我们描述了一个20岁的雌性猕猴(Macaca mulatta)的nk细胞淋巴增殖性疾病/白血病的病例。临床表现为轻度脾肿大和明显的淋巴细胞增多,血涂片可见小到中等淋巴细胞。流式细胞术和聚类分化检测淋巴细胞表面标志物cd3、cd8、cd4、cd20均为阴性;免疫组织化学显示细胞内存在CD3和颗粒酶b,这种免疫图谱与nk细胞表型一致。组织学上,这些细胞主要分布在脾红髓、肝窦内的血管内,骨髓内也有少量细胞。致癌病毒,如梅森-辉瑞猴病毒(MPMV);以前通常被称为猿猴逆转录病毒或SRV;反转录病毒科;反转录病毒群;猿猴免疫缺陷病毒;逆转录病毒科、慢病毒和灵长类嗜t淋巴病毒1型(PTLV1;俗称类人猿嗜t淋巴病毒1型,简称STLV1;该动物血清未检出逆转录病毒科Deltaretrovirus pritly1)。免疫组化应用EBNA2抗体检测恒河猴淋巴隐病毒(McGHV4/RLV)和食蟹猴淋巴隐病毒(McGHV10);正疱疹病毒科、淋巴隐病毒machine - egamma4和淋巴隐病毒machine - egamma13均为阴性。总之,这些发现与自然发生的nk细胞淋巴增生性疾病的诊断一致。据我们所知,以前在恒河猴中没有报道过nk细胞淋巴增生性疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.00
自引率
6.70%
发文量
127
审稿时长
6-16 weeks
期刊介绍: The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.
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