Daily variability of Pseudomonas aeruginosa density in cystic fibrosis sputum.

IF 5.4 2区 医学 Q1 RESPIRATORY SYSTEM
Lisa A Carmody, Lindsay J Caverly, Linda M Kalikin, Christina S Thornton, Richard H Simon, Donald R VanDevanter, John J LiPuma
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引用次数: 0

Abstract

Treatment-associated differences in Pseudomonas aeruginosa (Pa) density in sputum have been used as a response biomarker in clinical trials of cystic fibrosis (CF) therapies. Although most studies have included placebo-treated groups as comparators, variability of Pa density in untreated individuals has rarely been reported. We measured day-to-day differences in Pa density in 267 sputum sample pairs collected from 13 adults with CF during days in which no changes in antibiotic therapy occurred. Although the mean sputum Pa density change across all sample pairs was modest (-0.09 log10 16S rRNA gene copies/mL), variability in day-to-day changes were substantial (SD = 1.09) with one-quarter of sample pairs having >1 log10 differences in Pa density; approximately 8 % of pairs had >2 log10 differences in density. Day-to-day variability in Pa density differed substantially between study participants (p = .001). These results will support the design and interpretation of studies using sputum Pa density change as an efficacy biomarker.

囊性纤维化痰中铜绿假单胞菌密度的日变化。
在囊性纤维化(CF)治疗的临床试验中,痰中铜绿假单胞菌(Pa)密度的治疗相关差异已被用作反应性生物标志物。尽管大多数研究都将安慰剂治疗组作为比较,但未经治疗的个体中Pa密度的变异性很少有报道。在抗生素治疗未发生变化的日子里,我们测量了从13名CF成人中收集的267对痰样本中Pa密度的日常差异。尽管所有样本对的平均痰Pa密度变化不大(-0.09 log10 16S rRNA基因拷贝数/mL),但日常变化的可变性很大(SD = 1.09),四分之一的样本对的Pa密度差异为bb0.1 log10;大约8%的配对的密度差异为bb0.2 log10。研究参与者之间Pa密度的日常变化差异很大(p = .001)。这些结果将支持使用痰Pa密度变化作为疗效生物标志物的研究的设计和解释。
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来源期刊
Journal of Cystic Fibrosis
Journal of Cystic Fibrosis 医学-呼吸系统
CiteScore
10.10
自引率
13.50%
发文量
1361
审稿时长
50 days
期刊介绍: The Journal of Cystic Fibrosis is the official journal of the European Cystic Fibrosis Society. The journal is devoted to promoting the research and treatment of cystic fibrosis. To this end the journal publishes original scientific articles, editorials, case reports, short communications and other information relevant to cystic fibrosis. The journal also publishes news and articles concerning the activities and policies of the ECFS as well as those of other societies related the ECFS.
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