{"title":"A microcontroller-based system for flexible oxygen control in laboratory experiments.","authors":"Stefan Mucha","doi":"10.1242/jeb.249207","DOIUrl":null,"url":null,"abstract":"<p><p>Environmental control systems are important tools for experimental researchers studying animal-environment interactions. Commercial systems for the measurement and regulation of environmental oxygen conditions are relatively expensive and cannot always be adapted to varying experimental applications. Here, I present a low-cost and highly flexible oxygen control system using Arduino microcontrollers in combination with a commercial optical oxygen sensor. Hardware and software examples are provided for three different applications: single-setpoint, sequential and long-term dissolved oxygen (DO) control. All tested control systems created the desired DO conditions with high accuracy and repeatability across trials. The resources provided shown here can be adapted and modified to be used in a variety of experimental contexts.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744317/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1242/jeb.249207","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/7 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Environmental control systems are important tools for experimental researchers studying animal-environment interactions. Commercial systems for the measurement and regulation of environmental oxygen conditions are relatively expensive and cannot always be adapted to varying experimental applications. Here, I present a low-cost and highly flexible oxygen control system using Arduino microcontrollers in combination with a commercial optical oxygen sensor. Hardware and software examples are provided for three different applications: single-setpoint, sequential and long-term dissolved oxygen (DO) control. All tested control systems created the desired DO conditions with high accuracy and repeatability across trials. The resources provided shown here can be adapted and modified to be used in a variety of experimental contexts.
期刊介绍：
Journal of Experimental Biology is the leading primary research journal in comparative physiology and publishes papers on the form and function of living organisms at all levels of biological organisation, from the molecular and subcellular to the integrated whole animal.
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