Harnessing Trichoderma asperellum: Tri-Trophic Interactions for Enhanced Black Gram Growth and Root Rot Resilience

IF 3.5 4区 生物学 Q2 MICROBIOLOGY
Praveen Vijayakumar, Sudha Appusami, Selva Amala Anbazhagan, Ramjegathesh Rajendran, Kavitha Shanmugam, Kahkashan Perveen, Najat A. Bukhari, Riyaz Sayyed
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引用次数: 0

Abstract

Root rot caused by Macrophomina phaseolina, a common soil-borne disease in black gram, is managed with chemical fungicides, leading to toxicity and degradation of beneficial soil microbes. Existing bioagents, like talc formulation, cause leaching, clogging, and reduced productivity. The development of liquid bio-formulation via drip irrigation is crucial to mitigate biotic stress and maximize yield. This study aims to investigate the efficacy and survivability of liquid formulation of Trichoderma asperellum against root rot and its growth promotion. The results showed that Tv1 effectively inhibited M. phaseolina (66.67%), under in vitro condition. The vigor index of 4025.00 and the spore load of 1 × 108 cfu/mL were recorded from plant growth promotion and spermosphere study @ 5 mL/kg of seeds with formulation. The study found that combined application of seed treatment @ 5 mL/kg of seed and soil application @ 10 mL/L of water significantly reduced disease incidence (9.1%) against control (74.3%), with increased biomass index. There are 32 mVOCs profiled during the tritrophic interaction in roots of black gram and they were up or downregulated, viz., mollugin, pentadecanoic acid, cyclopropaneoctanoic acid, 2-octyl-, methyl ester, rhodopin, dodecanoic acid, 1,2,3-propanetriyl ester by involved in defense mechanism and biosynthetic pathways like jasmonic acid, glyconeogenic and act as acyl-CoA: acyltransferase 2 inhibitor. The results of this study confirmed that liquid formulation performs better in growth promotion, survivability on seed surface, and managing root rot of black gram compared talc-based formulation.

利用曲霉木霉:三营养相互作用提高黑革生长和根腐病恢复能力。
黑革中一种常见的土传病害——phaseolina引起的根腐病,使用化学杀菌剂进行治理,导致有益土壤微生物的毒性和降解。现有的生物制剂,如滑石粉制剂,会导致浸出、堵塞和降低生产率。通过滴灌技术开发液体生物制剂是缓解生物胁迫和实现产量最大化的关键。本研究旨在探讨曲霉木霉液剂对根腐病的防治效果、成活能力及对其生长的促进作用。结果表明,在体外条件下,Tv1对菜绿分枝杆菌的抑制率为66.67%。在5 mL/kg剂量下对种子进行生长促进和孢粉负荷研究,测定了活力指数为4025.00,孢子负荷为1 × 108 cfu/mL。研究发现,种子处理量为5 mL/kg,土壤处理量为10 mL/L,与对照(74.3%)相比,病害发生率显著降低(9.1%),生物量指数显著提高。黑克根在三营养相互作用过程中共检测到32种主要挥发性有机化合物(mollugin、pentocanoic acid、cyclopropane辛酸、2-辛烷酸、甲酯、rhodopin、十二烷酸、1,2,3-propanetriyl ester),它们参与茉莉酸、糖异生等防御机制和生物合成途径,并作为酰基辅酶a:酰基转移酶2抑制剂。本研究结果证实,液体配方比滑石粉配方在促进黑克生长、种子表面存活率和根腐病防治方面具有更好的效果。
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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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