Supplementation of recombinant human lysozyme into diets affects the growth performance, muscle quality, immunity and intestinal microbiota in large yellow croaker Larimichthys crocea

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-02-01 DOI:10.1016/j.fsi.2024.110062

Dayin Liu , Peng Qu , Yingxu Huangfu , Xinyu Wang , Mingzhu Pan , Yang Wu , Kangsen Mai , Wenbing Zhang

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引用次数: 0

Abstract

The aim of the present study was to investigate the influence of supplementation of lysozyme (LZM) into diet on the growth performance, muscle quality, immunity, intestinal microbiota in large yellow croaker (Larimichthys crocea) with initial body weight of 194.5 ± 0.27 g. After a 70-day feeding trial, 6 experimental diets with LZM supplementation at 0 (LZM0), 10 (LZM10), 30 (LZM30), 50 (LZM50), 70 (LZM70) and 90 mg/kg (LZM90) were tested. Results showed that the fish in the LZM70 group exhibited the lowest feed conversion ratio and the highest weight gain (WG), along with the highest trypsin and Na⁺/K⁺ ATPase activities in intestine (P < 0.05). The LZM activity in serum and intestine was significantly reduced in all dietary LZM supplemented groups compared to the LZM0 group (P < 0.05). Compared with that in the LZM0 group, the gene expressions of claudin 11, bcl-2, nlrp 3, tnf α, il-10 and tgf β in intestine in the LZM90 group were significantly elevated, while bax and caspase3 were significantly downregulated (P < 0.05). Meanwhile, the group supplemented with 90 mg/kg of dietary LZM also increased muscle crude lipid content, springiness and drip loss, along with decreased crude protein content, shear force and hardness compared with other groups (P < 0.05). Furthermore, the results of intestinal microbiota showed that compared to those in the LZM0 group, relative abundances of Fusobacterium in the LZM30 and LZM90 groups were decreased, and the relative abundances of Achromobacter, Mycoplasma and Cetobacterium were increased. In conclusion, appropriate supplementation of LZM in diet promoted the growth performance, improved immunity, adjusted intestinal microbiota and muscle quality of large yellow croaker. Furthermore, the optimal level of dietary LZM supplementation for large yellow croaker was estimated to be 67.14 mg/kg based on the quadratic regression for WG.

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饲料中添加重组人溶菌酶对大黄鱼生长性能、肌肉品质、免疫力和肠道菌群有影响。

本试验旨在研究日粮中添加溶菌酶（LZM）对初始体重为194.5±0.27 g的大黄鱼生长性能、肌肉品质、免疫力和肠道菌群的影响。饲喂70 d后，分别在6种试验饲粮中添加0 （LZM0）、10 （LZM10）、30 （LZM30）、50 （LZM50）、70 （LZM70）和90 mg/kg （LZM90）的LZM。结果表明：LZM70组饲料系数最低，增重最高，肠道胰蛋白酶和Na+/K+ atp酶活性最高（P < 0.05）；与LZM0组相比，各饲粮添加LZM组血清和肠道中LZM活性显著降低（P < 0.05）。与LZM0组比较，LZM90组小鼠肠组织中claudin 11、bcl-2、nlrp 3、tnf α、il-10、tgf β基因表达量显著升高，bax、caspase3基因表达量显著下调（P < 0.05）。同时，与其他组相比，饲粮中添加90 mg/kg LZM组肌肉粗脂肪含量、弹性和滴水损失显著增加（P < 0.05），粗蛋白质含量、剪切力和硬度显著降低（P < 0.05）。此外，肠道菌群结果显示，与LZM0组相比，LZM30和LZM90组的梭杆菌相对丰度降低，无色杆菌、支原体和Cetobacterium相对丰度升高。综上所述，饲粮中适当添加LZM可促进大黄鱼的生长性能，提高免疫力，调节肠道菌群和肌肉品质。通过二次回归分析，得出大黄鱼饲粮中LZM的适宜添加量为67.14 mg/kg。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.